Lhuguenot J C, Maume B F
Biomed Mass Spectrom. 1980 Nov;7(11-12):529-32. doi: 10.1002/bms.1200071115.
Extracts have been made from culture medium of rat medullar adrenal cells developed in tissue culture in this laboratory. After pentafluorobenzylimine-trimethylsilyl ether formation the catecholamine derivatives were characterized by gas-liquid chromatography chemical ionization mass spectrometry. In order to assess the catecholamine production capabilities of the cells in culture, a mass spectrometric method with isotope dilution has been devised. Chemical ionization selected ion monitoring allows specific detection at the nanogram level in a higher mass range (400-600 amu) than in the electron impact mode. The isotopic dilution method with 14C catecholamines gives rise to accurate measurements and linear response in the picomole range. The use of the [M-15]+ ion for monitoring m/z values minimizes errors in selected ion monitoring analysis. The results obtained are computerized and treated by the data system for fine background subtraction when high sensitivity and accuracy are required.
从本实验室组织培养的大鼠肾上腺髓质细胞的培养基中提取了提取物。在形成五氟苄基亚胺-三甲基硅醚后，通过气-液色谱化学电离质谱法对儿茶酚胺衍生物进行了表征。为了评估培养细胞产生儿茶酚胺的能力，设计了一种同位素稀释质谱法。化学电离选择离子监测能够在比电子轰击模式更高的质量范围（400-600原子质量单位）内以纳克水平进行特异性检测。用14C儿茶酚胺的同位素稀释法可在皮摩尔范围内进行准确测量并得到线性响应。使用[M-15]+离子监测m/z值可将选择离子监测分析中的误差降至最低。当需要高灵敏度和高精度时，所获得的结果由数据系统进行计算机处理并进行精细的背景扣除。
