Rubinow A, Skinner M, Cohen A S
Circulation. 1981 Jun;63(6):1285-8. doi: 10.1161/01.cir.63.6.1285.
Digoxin (5 mg/ml) was added to 10-mg and 20-mg pellets of purified primary and secondary amyloid fibrils, a normal human liver and heart homogenate and a homogenate from the heart of a patient with amyloid cardiomyopathy who had not received digitalis. After centrifugation, the supernatants were recovered and assayed for digoxin concentrations. Aliquots from the sediments were studied for the presence of digoxin, using rabbits antidigoxin antiserum and an indirect immunofluorescent technique. The results showed that 0.11--0.13 ng/ml of digoxin bound per milligram of fibrils and could not be separated by repeated washings. Elution with citrate or changes in the pH of the buffer. Immunofluorescent studies demonstrated diffusely bright immunofluorescence with the fibril preparation and amyloid heart homogenate when reacted with digoxin and digoxin-specific antiserum. These studies demonstrate that isolated amyloid fibrils bind digoxin and suggest that this interaction may play some role in the sensitivity to digitalis that has been observed in some patients with amyloid cardiomyopathy.
将地高辛（5毫克/毫升）添加到纯化的原发性和继发性淀粉样原纤维的10毫克和20毫克丸剂、正常人肝脏和心脏匀浆以及一名未接受洋地黄治疗的淀粉样心肌病患者的心脏匀浆中。离心后，回收上清液并测定地高辛浓度。使用兔抗地高辛抗血清和间接免疫荧光技术研究沉淀物的等分试样中是否存在地高辛。结果表明，每毫克原纤维结合0.11--0.13纳克/毫升地高辛，且经反复洗涤无法分离。用柠檬酸盐洗脱或改变缓冲液的pH值。免疫荧光研究表明，当与地高辛和地高辛特异性抗血清反应时，原纤维制剂和淀粉样心脏匀浆呈现弥漫性明亮的免疫荧光。这些研究表明，分离出的淀粉样原纤维结合地高辛，并提示这种相互作用可能在一些淀粉样心肌病患者中观察到的对洋地黄的敏感性中起某种作用。
