Cornwell G G, Westermark P, Natvig J B, Murdoch W
Immunology. 1981 Nov;44(3):447-52.
Antiserum specific for human prealbumin (HPA) was studied by indirect immunofluorescence on tissue sections of cardiac ventricles containing senile cardiac amyloid. The pattern of reactivity was identical to that previously reported for an antiserum specific for protein ASc1 (formerly designated ASCA present in these tissues. Anti-HPA failed to react with isolated atrial amyloid (IAA), primary amyloid (A lambda I, A lambda IV, A lambda VI), secondary amyloid (AA), amyloid associated with medullary carcinoma of the thyroid (AEt), pancreatic amyloid associated with adult onset diabetes, cerebral amyloid present in Alzheimer's disease or lichen amyloid. THe reaction of anti-HPA was completely blocked by purified human prealbumin but was not influenced by absorption with purified human albumin or proteins extracted from any amyloid types tested. The anti-HPA reaction was also completely blocked by purified protein ASc1, and the reaction of anti-ASc1 was similarly blocked by purified HPA. These studies suggest that senile cardiac amyloid of the ASc1 type contains prealbumin or a protein antigenically closely related to this molecule.
通过间接免疫荧光法，在含有老年心脏淀粉样蛋白的心室组织切片上研究了针对人前白蛋白（HPA）的抗血清。反应模式与先前报道的针对这些组织中存在的蛋白ASc1（以前称为ASCA）的抗血清的反应模式相同。抗HPA未能与分离的心房淀粉样蛋白（IAA）、原发性淀粉样蛋白（AλI、AλIV、AλVI）、继发性淀粉样蛋白（AA）、与甲状腺髓样癌相关的淀粉样蛋白（AEt）、与成人发病型糖尿病相关的胰腺淀粉样蛋白、阿尔茨海默病中存在的脑淀粉样蛋白或苔藓样淀粉样蛋白发生反应。纯化的人前白蛋白可完全阻断抗HPA的反应，但不受纯化的人白蛋白或从任何测试的淀粉样蛋白类型中提取的蛋白质吸收的影响。抗HPA反应也被纯化的蛋白ASc1完全阻断，抗ASc1的反应同样被纯化的HPA阻断。这些研究表明，ASc1型老年心脏淀粉样蛋白含有前白蛋白或与该分子抗原性密切相关的蛋白质。
