Heikinheimo M, Wahlström T, Aula P, Virtanen I, Seppälä M
J Clin Endocrinol Metab. 1980 Dec;51(6):1432-6. doi: 10.1210/jcem-51-6-1432.
The synthesis of pregnancy-specific beta-1-glycoprotein (SP1) was studied in amniotic fluid cell cultures using RIA, immunoperoxidase, and immunofluorescence techniques. SP1 was found by RIA in all 11 sonicates and in 21 of 26 culture media. The SP1-immunoreactive material was immunologically similar to maternal serum SP1. Immunoperoxidase and indirect immunofluorescence staining were positive in large cells identified as epithelial amniotic cells by labeling with antikeratin antibodies. Fibroblast-like cells were occasionally found in cultures, but they did not contain demonstrable amounts of SP1. The physiological significance of the findings presented remains unclear.
采用放射免疫分析法（RIA）、免疫过氧化物酶法和免疫荧光技术，对羊水细胞培养物中妊娠特异性β1-糖蛋白（SP1）的合成进行了研究。通过RIA在所有11份超声处理液以及26份培养基中的21份中检测到了SP1。SP1免疫反应性物质在免疫学上与母体血清SP1相似。用抗角蛋白抗体标记后，鉴定为羊膜上皮细胞的大细胞中，免疫过氧化物酶和间接免疫荧光染色呈阳性。培养物中偶尔会发现成纤维细胞样细胞，但它们不含可检测到的SP1。所呈现结果的生理学意义仍不清楚。
