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黏附在囊性纤维化患者铜绿假单胞菌肺部感染发病机制中的作用。

Role of adherence in the pathogenesis of Pseudomonas aeruginosa lung infection in cystic fibrosis patients.

作者信息

Woods D E, Bass J A, Johanson W G, Straus D C

出版信息

Infect Immun. 1980 Dec;30(3):694-9. doi: 10.1128/iai.30.3.694-699.1980.

Abstract

A correlation has been demonstrated between the in vitro adherence of Pseudomonas aeruginosa to upper respiratory tract epithelium and colonization of the respiratory tract by this organism. Twenty patients with cystic fibrosis (CF) and 20 age-matched controls were examined in this study. All of the CF patients but none of the controls were colonized with P. aeruginosa at the time of study. P. aeruginosa adherence to isolated epithelial cells, as determined by an in vitro assay, was 19.1 +/- 1.1 bacteria per buccal epithelial cell in the CF patients and 2.3 +/- 0.3 bacteria per cell in the controls (P < 0.01). P. aeruginosa strains of the mucoid colony type adhered in significantly lower numbers to buccal epithelial cells than did strains of the rough colony type (1.8 + 0.1 versus 24.8 +/- 0.9, P < 0.001). This difference might explain the common observation that the initial pseudomonas colonization of the respiratory tract of CF patients is due to organisms of the rough colony type. We have further demonstrated that increased P. aeruginosa adherence in vitro varies directly with the loss of a protease-sensitive glycoprotein, fibronectin, from the cell surface, as well as increased levels of salivary proteases in CF patients. When examined by a direct radioimmune binding assay, buccal cells from CF patients possessed only 17% of the total cell surface fibronectin present on similar cells obtained from controls. Salivary protease levels, as measured by (125)I release from an (125)I-labeled insoluble fibrin matrix, were increased about threefold in CF patients versus controls. Thus, colonization of the respiratory tract by P. aeruginosa in CF patients correlates well with buccal cell adherence of this organism; increased adherence is associated with decreased amounts of fibronectin on respiratory epithelial cell surfaces and increased levels of salivary proteases.

摘要

已证实铜绿假单胞菌在体外对上呼吸道上皮的黏附与该菌在呼吸道的定植之间存在相关性。本研究对20例囊性纤维化(CF)患者和20例年龄匹配的对照者进行了检查。在研究时,所有CF患者均被铜绿假单胞菌定植,而对照者无一被定植。通过体外试验测定,CF患者中铜绿假单胞菌对分离的上皮细胞的黏附为每颊黏膜上皮细胞19.1±1.1个细菌,对照者为每细胞2.3±0.3个细菌(P<0.01)。黏液型菌落的铜绿假单胞菌菌株对颊黏膜上皮细胞的黏附数量明显低于粗糙型菌落菌株(1.8±0.1对24.8±0.9,P<0.001)。这种差异可能解释了常见的观察结果,即CF患者呼吸道最初的假单胞菌定植是由粗糙型菌落的细菌引起的。我们进一步证明,体外铜绿假单胞菌黏附增加与细胞表面一种蛋白酶敏感糖蛋白纤连蛋白的丢失直接相关,也与CF患者唾液蛋白酶水平升高有关。通过直接放射免疫结合试验检测时,CF患者的颊细胞仅含有从对照者获得的类似细胞上存在的总细胞表面纤连蛋白的17%。以从125I标记的不溶性纤维蛋白基质释放125I来测量,CF患者的唾液蛋白酶水平比对照者增加了约三倍。因此,CF患者呼吸道被铜绿假单胞菌定植与该菌对颊细胞的黏附密切相关;黏附增加与呼吸道上皮细胞表面纤连蛋白量减少和唾液蛋白酶水平升高有关。

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