Nolte F S, Kapral F A
Infect Immun. 1981 Mar;31(3):1251-60. doi: 10.1128/iai.31.3.1251-1260.1981.
Studies were conducted to determine the immunogenicity of purified Staphylococcus aureus delta-toxin. Rabbits and guinea pigs immunized with delta-toxin incorporated into a multiple antibody, whereas animals given toxin in saline or toxin in saline with Tween 80 did not produce antibody. The immunoglobulin G (IgG) fraction isolated by chromatography on protein A-Sepharose was examined for the presence of anti-delta-toxin antibody by immunoelectrophoresis, immunodiffusion, quantitative precipitation tests, affinity chromatography, and toxin neutralization tests. Although delta-toxin-specific IgG precipitated the toxin in agar gels, the antibody did not neutralize the toxin's hemolytic activity. Delta-toxin binding to human erythrocyte membranes was demonstrated by indirect immunofluorescent staining of toxin-treated erythrocytes.
开展了多项研究以确定纯化的金黄色葡萄球菌δ毒素的免疫原性。用掺入多种抗体中的δ毒素免疫的兔子和豚鼠产生了抗体，而给予盐水中的毒素或盐水中含吐温80的毒素的动物则未产生抗体。通过在蛋白A-琼脂糖凝胶上进行色谱分离得到的免疫球蛋白G（IgG）组分，通过免疫电泳、免疫扩散、定量沉淀试验、亲和色谱和毒素中和试验检测抗δ毒素抗体的存在。尽管δ毒素特异性IgG在琼脂凝胶中沉淀了毒素，但该抗体并未中和毒素的溶血活性。通过对毒素处理的红细胞进行间接免疫荧光染色，证明了δ毒素与人红细胞膜的结合。
