通过使用抗原-抗体复合物中的人免疫球蛋白G的固相放射免疫测定法测定人免疫球蛋白M类风湿因子。
Determination of human immunoglobulin M rheumatoid factor by a solid-phase radioimmunoassay which uses human immunoglobulin G in antigen-antibody complexes.
作者信息
Ziola B, Meurman O, Matikainen M T, Salmi A, Kalliomäki J L
出版信息
J Clin Microbiol. 1978 Aug;8(2):134-41. doi: 10.1128/jcm.8.2.134-141.1978.
Abstract
A solid-phase radioimmunoassay for the rapid determination of human immunoglobulin M (IgM) rheumatoid factor (RF) has been developed. Preparation of the solid phase for the assay involved the formation of complexes between respiratory syncytial virus-specific human IgG antibodies and virus antigen on the surface of polystyrene balls. Binding of serum RF to IgG in the immune complex was subsequently detected by 125I-labeled mu-chain-specific antibodies to human IgM. The amount of radioactive indicator antibody bound was converted to units of RF by comparison to the standard curve for an RF reference-serum pool. This assay should prove useful in studies of the physiological role of RF, since it can effectively measure low levels of circulating RF.
摘要
已开发出一种用于快速测定人免疫球蛋白M（IgM）类风湿因子（RF）的固相放射免疫测定法。该测定法的固相制备涉及呼吸道合胞病毒特异性人IgG抗体与聚苯乙烯球表面病毒抗原之间形成复合物。随后通过125I标记的人IgMμ链特异性抗体检测血清RF与免疫复合物中IgG的结合。通过与RF参考血清库的标准曲线比较，将结合的放射性指示抗体量换算为RF单位。该测定法在RF生理作用的研究中应会证明有用，因为它能有效测量循环中低水平的RF。
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本文引用的文献
1
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2
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Nature. 1962 May 5;194:495-6. doi: 10.1038/194495a0.
3
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Acta Rheumatol Scand Suppl. 1965:SUPPL 9:1-121. doi: 10.3109/rhe1.1965.10.suppl-9.01.
4
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Ann Med Exp Biol Fenn. 1961;39 (Suppl 7):1-60.
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6
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8
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