用于鉴定无乳链球菌（兰斯菲尔德B组）的快速试管CAMP试验
Rapid tube CAMP test for identification of Streptococcus agalactiae (Lancefield group B).
作者信息
Phillips E A, Tapsall J W, Smith D D
出版信息
J Clin Microbiol. 1980 Aug;12(2):135-7. doi: 10.1128/jcm.12.2.135-137.1980.
Abstract
A rapid CAMP test for the presumptive identification of Streptococcus agalactiae (Lancefield group B) is described. Sheep erythrocytes, sensitized by staphylococcal beta-lysin and suspended in phosphate-buffered saline, were used to determine the lytic capacity of the neutralized supernatant fluids of 4-h broth cultures of streptococci being tested. A total of 96.2% of 130 group B streptococci gave positive CAMP tests, that is, lysis of the sheep erythrocytes after 10 min of exposure of streptococcal supernatants, whereas none of 381 non-group B streptococci tested produced any lysis. The test described provides presumptive identification of group B streptococci within 4 h and eliminates problems of intermediate reactions so that a positive test is indicative only of CAMP factor production.
摘要
本文描述了一种用于初步鉴定无乳链球菌（兰斯菲尔德B组）的快速CAMP试验。用经葡萄球菌β-溶血素致敏并悬浮于磷酸盐缓冲盐水中的绵羊红细胞，来测定受试链球菌4小时肉汤培养物的中和上清液的溶血能力。130株B组链球菌中，共有96.2%的菌株CAMP试验呈阳性，即链球菌上清液作用10分钟后绵羊红细胞发生溶血，而381株非B组链球菌均未产生任何溶血现象。所描述的该试验可在4小时内初步鉴定B组链球菌，并消除了中间反应的问题，因此阳性试验仅表明产生了CAMP因子。
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