Facklam R R, Padula J F, Wortham E C, Cooksey R C, Rountree H A
J Clin Microbiol. 1979 Jun;9(6):665-72. doi: 10.1128/jcm.9.6.665-672.1979.
Several presumptive tests were evaluated for their effectiveness in differentiating streptococci. When the tests were combined into a battery and the resulting reactions were interpreted as patterns, the overall presumptive identification rate was at least 97%. We used the hemolytic reaction, susceptibility to bacitracin and sulfamethoxazole plus trimethoprim (1.25 micrograms plus 23.75 micrograms), and standard CAMP reactions on sheep blood Trypticase soy agar, and bile-esculin and 6.5% NaCl agar tolerance tests with incubation in candle extinction jars. Subsequently, 98.9% of the group A; 95.3% of the group B; 100% of the beta-hemolytic non-group A, B, or D; 92.3% of group D enterococcal; 100% of the group D non-enterococcal; and 92.8% of the viridans streptococci were presumptively identified. We then used the hemolytic reactions, susceptibility of bacitracin and sulfamethoxazole-plus-trimethoprim disks, CAMP disk reactions on sheep blood Trypticase soy agar and bile-esculin and 6.5% NaCl agar tolerance tests with incubation in normal atmosphere. Subsequently, 98.1% of the group A; 98.6% of the group B; 99.2% of the beta hemolytic non-group A, B, or D; 97.5% of the group D entercoccal; 97.6% of the group D non-entercoccal; and 92.4% of the viridans strains were presumptively identified.
对几种用于鉴别链球菌的推测性试验的有效性进行了评估。当将这些试验组合成一套并将所得反应解释为模式时，总体推测性鉴定率至少为97%。我们使用了溶血反应、对杆菌肽以及磺胺甲恶唑加甲氧苄啶（1.25微克加23.75微克）的敏感性、在绵羊血胰蛋白胨大豆琼脂上的标准CAMP反应，以及在烛缸中培养的胆汁-七叶苷和6.5%氯化钠琼脂耐受性试验。随后，A组的98.9%；B组的95.3%；β溶血非A、B或D组的100%；D组肠球菌的92.3%；D组非肠球菌的100%；以及草绿色链球菌的92.8%被推测性鉴定。然后，我们使用了溶血反应、杆菌肽和磺胺甲恶唑-甲氧苄啶纸片的敏感性、在绵羊血胰蛋白胨大豆琼脂上的CAMP纸片反应以及在正常大气中培养的胆汁-七叶苷和6.5%氯化钠琼脂耐受性试验。随后，A组的98.1%；B组的98.6%；β溶血非A、B或D组的99.2%；D组肠球菌的97.5%；D组非肠球菌的97.6%；以及草绿色链球菌菌株的92.4%被推测性鉴定。
