Wilkinson H W
J Clin Microbiol. 1977 Jul;6(1):42-5. doi: 10.1128/jcm.6.1.42-45.1977.
The modification of the CAMP test for goup B streptococci involved substituting a paper disk impregnated with partially purified beta-hemolysin for the staphylococcal culture that was the source of beta-hemolysin in the original test. The disk is placed onto a sheep blood agar plate beside the streak of Streptococcus being tested. The plate is then incubated aerobically at 35 degrees C. A positive reaction consists of a lunar-shaped clear zone that appears within 24 h in the dark beta-hemolysin zone surrounding the disk. A double-blind study of 135 randomly coded streptococcal isolates showed complete agreement between the CAMP-disk test and the standard Lancefield precipitin test. All group B streptococci tested had positive reactions, and all strains tested from streptococcal groups A, C, D, and G were negative. The CAMP-disk test is a simple and convenient way to identify presumptively group B streptococci.
对B群链球菌的CAMP试验进行的改进包括，用浸有部分纯化的β-溶血素的纸片替代原始试验中作为β-溶血素来源的葡萄球菌培养物。将该纸片放置在待检测的链球菌划线旁的羊血琼脂平板上。然后将平板在35℃有氧条件下孵育。阳性反应表现为在纸片周围的暗β-溶血素区域内24小时内出现的新月形透明区。一项对135株随机编码的链球菌分离株的双盲研究表明，CAMP纸片试验与标准的兰斯菲尔德沉淀试验完全一致。所有检测的B群链球菌均呈阳性反应，而从A、C、D和G群链球菌检测的所有菌株均为阴性。CAMP纸片试验是一种简单便捷的方法，可用于初步鉴定B群链球菌。
