批量分离的小鼠少突胶质细胞的免疫学特性。
Immunological properties of bulk isolated mouse oligodendrocytes.
作者信息
Bologa-Sandru L, Siegrist H P, Herschkowitz N
出版信息
Neurosci Lett. 1981 Mar 10;22(2):131-5. doi: 10.1016/0304-3940(81)90076-8.
Mouse oligodendrocytes were isolated in bulk. These cells were 95% galactocerebroside-positive. Rabbit anti-mouse oligodendrocyte antisera reacted in radioimmunoassay and in indirect immunofluorescence with mouse oligodendrocytes in suspension or in culture. The activity of the sera was not blocked by adsorption with galactocerebroside or by preincubation of cells with anti-galactocerebroside serum, although it cross-reacted with galactocerebroside in a serological test. The staining was not impaired by the pretreatment of cells with trypsin, neuraminidase or acetone, nor by adsorption of serum with myelin basis protein.
批量分离出小鼠少突胶质细胞。这些细胞95%呈半乳糖脑苷脂阳性。兔抗小鼠少突胶质细胞抗血清在放射免疫测定以及间接免疫荧光中，与悬浮或培养状态下的小鼠少突胶质细胞发生反应。血清活性不会因用半乳糖脑苷脂吸附或细胞用抗半乳糖脑苷脂血清预孵育而被阻断，尽管它在血清学检测中与半乳糖脑苷脂发生交叉反应。用胰蛋白酶、神经氨酸酶或丙酮对细胞进行预处理，或者用髓磷脂碱性蛋白吸附血清，均不会损害染色效果。
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