在小鼠脑细胞培养物中观察到的发育过程中半乳糖脑苷脂含量的相对变化。

Relative changes in the amount of galactocerebroside during development as observed in mouse brain cell cultures.

作者信息

Bologa L, Siegrist H P, Herschkowitz N

出版信息

Neurosci Lett. 1981 Nov 4;26(3):335-9. doi: 10.1016/0304-3940(81)90155-5.

Abstract

Relative changes in the amount of galactocerebroside (GC) during development were measured in mouse brain cell cultures at different stages of development. For this purpose we used an 125I-labelled protein A indirect assay modified in the respect that the total amount of cellular proteins was evaluated before counting the radioactivity. The amount of GC greatly increased between the 10th and the 14th day of culture, then a steady state was reached between the 14th and the 20th day of culture. This change correlates well with the dynamics of the number of oligodendrocytes we observed earlier. These data suggests that the increase of the GC amount in culture during development corresponds to the increase in the number of GC-positive oligodendrocytes rather than to the increase in the number of GC molecules per cell.

摘要

在小鼠脑细胞培养物中，于不同发育阶段测量了半乳糖脑苷脂（GC）含量的相对变化。为此，我们使用了一种经改进的¹²⁵I标记蛋白A间接测定法，即在计数放射性之前先评估细胞总蛋白量。培养第10天至第14天期间，GC含量大幅增加，然后在培养第14天至第20天达到稳定状态。这种变化与我们之前观察到的少突胶质细胞数量动态变化密切相关。这些数据表明，发育过程中培养物中GC含量的增加对应于GC阳性少突胶质细胞数量的增加，而非每个细胞中GC分子数量的增加。