Ishaque M
Centre de Recherche en Microbiologie Appliquée, Institut Armand-Frappier, Université du Québec, Laval-des-Rapides, Canada.
Cytobios. 1992;71(284):19-27.
Oxidation of palmitate by Mycobacterium lepraemurium isolated from C3H mice lepromata (in vivo) and also grown on Ogawa egg-yolk medium (in vitro) was investigated. Palmitate was found to be oxidized, after a lag period of about 8 h, by both the in vivo and in vitro grown bacilli. Cell-free extracts prepared from in vivo and in vitro grown cells catalysed an active oxidation of palmitate after a lag period of 3-4 h. The amount of ATP increased, with the increase in time during oxidation of palmitate by the cell-free extracts. The generation of ATP was strongly inhibited by the inhibitors rotenone, antimycin A and cyanide as well as by the uncouplers 2,4-dinitrophenol and 2,6-dibromophenol. These results indicated that oxidation of palmitate by the in vivo and in vitro grown M. lepraemurium is mediated through the respiratory chain using oxygen as the terminal electron acceptor.
对从C3H小鼠麻风瘤中分离出的（体内）以及在小川蛋黄培养基上培养的（体外）鼠麻风分枝杆菌对棕榈酸的氧化作用进行了研究。发现棕榈酸在约8小时的延迟期后，被体内和体外培养的杆菌氧化。从体内和体外培养的细胞制备的无细胞提取物在3 - 4小时的延迟期后催化了棕榈酸的活性氧化。在无细胞提取物氧化棕榈酸的过程中，ATP的量随着时间的增加而增加。ATP的生成受到抑制剂鱼藤酮、抗霉素A和氰化物以及解偶联剂2,4 - 二硝基苯酚和2,6 - 二溴苯酚的强烈抑制。这些结果表明，体内和体外培养的鼠麻风分枝杆菌对棕榈酸的氧化是通过以氧作为末端电子受体的呼吸链介导的。
