Kato L, Ishaque M
Cytobios. 1975;12(45):31-43.
Particles from Mycobacterium lepraemurium catalysed the oxidation of NADH with oxygen as the terminal electron acceptor. The preparations contained cytochromes of the a + a3'b and c types, as well as CO-binding pigments. The NADH oxidase activity was sensitive to inhibitors of the flavoprotein system as well as to HQNO and antimycin A. In addition, a cytochrome oxidase sensitive to cyanide was also present. The system was inhibited by the thiol-binding agent, PCMB, and thus indicated the involvement of sulphydryl group in the enzymatic oxidation of NADH. The sensitivity of the NADH oxidase system to all the inhibitors of the respiratory chain and the effect of these inhibitors on the absorption spectra suggested that cytochromes of the b, c, a + a3 types are involved in the transfer of electrons in NADH oxidation.
来自鼠麻风杆菌的颗粒以氧气作为末端电子受体催化NADH的氧化。制剂中含有a + a3'b和c型细胞色素，以及一氧化碳结合色素。NADH氧化酶活性对黄素蛋白系统抑制剂以及HQNO和抗霉素A敏感。此外，还存在对氰化物敏感的细胞色素氧化酶。该系统被巯基结合剂PCMB抑制，因此表明巯基参与了NADH的酶促氧化。NADH氧化酶系统对呼吸链所有抑制剂的敏感性以及这些抑制剂对吸收光谱的影响表明，b、c、a + a3型细胞色素参与了NADH氧化过程中的电子传递。
