Fagelman K M, Flint L M, McCoy M T, Polk H C, Trachtenberg L S
Arch Surg. 1981 Jun;116(6):761-4. doi: 10.1001/archsurg.1981.01380180021005.
Experiments were conducted to ascertain whether nonspecific host defenses could be enhanced in a reliable animal model simulating the local bacterial infection that frequently complicates surgical wounds. The test lesion was studied in detail and exemplifies the concept that the ultimate expression of the host-pathogen interaction is the capacity of that pathogen to persist or grow in a given host. Mice were challenged by intramuscular insertion of cotton suture impregnated with 10(7) to 10(8) Escherichia coli K-12. The mice were subsequently killed at intervals, and the suture and muscle mass were retrieved, homogenized, and quantitatively cultured. Numbers of viable organisms in tissue from control animals were compared with those from experimental animals that received BCG (Bacillus Calmette-Guérin) vaccine, a nonspecific immunostimulant, prior to bacterial challenge. Improved tissue antibacterial activity appeared in animals that had received BCG vaccine 13 days prior to bacterial challenge. Differing doses and intervals were not protective. Enhancement of nonspecific host defense mechanisms may be helpful in combination with current measures for improved control of surgical wound infection.
开展了多项实验，以确定在模拟经常使手术伤口复杂化的局部细菌感染的可靠动物模型中，非特异性宿主防御功能是否能够得到增强。对测试损伤进行了详细研究，该损伤例证了宿主与病原体相互作用的最终表现是该病原体在特定宿主中持续存在或生长的能力这一概念。通过肌肉注射浸渍有10(7)至10(8)大肠杆菌K-12的棉缝线对小鼠进行攻击。随后每隔一段时间处死小鼠，取出缝线和肌肉组织，进行匀浆并进行定量培养。将对照动物组织中的活菌数量与在细菌攻击前接受卡介苗（Bacillus Calmette-Guérin）疫苗（一种非特异性免疫刺激剂）的实验动物组织中的活菌数量进行比较。在细菌攻击前13天接受卡介苗疫苗的动物中，组织抗菌活性有所提高。不同的剂量和间隔时间并无保护作用。增强非特异性宿主防御机制可能有助于结合当前措施，更好地控制手术伤口感染。
