Bentley S A, Alabaster O, Foidart J M
Br J Haematol. 1981 Jun;48(2):287-91.
Paraffin embedded sections of formalin fixed, decalcified, normal, human, vertebral bone were stained immunohistochemically for collagen types I, III and IV using the peroxidase--anti-peroxidase (PAP) technique. Preparations stained for collagen types I and III were virtually identical in appearance. These substrates were localized to the cytoplasm and fibrillar processes of a population of cells which were sparsely distributed within the haemopoietic compartment of the bone marrow, being particularly prominent in relation to the marrow sinusoids and fat spaces. They would thus appear to parallel the known distributions of reticulum cells, although their morphology differed in some respects from classical descriptions of the latter cell type. Type IV collagen was found in association with the endothelial lining of the sinusoids. Other connective tissue elements (bone, periosteum, endosteum, blood vessels, etc.) showed characteristic collagen heterogeneity. These results indicate that collagen is a significant component of the bone marrow connective tissue.
采用过氧化物酶-抗过氧化物酶（PAP）技术，对经福尔马林固定、脱钙的正常人类椎骨石蜡包埋切片进行Ⅰ型、Ⅲ型和Ⅳ型胶原的免疫组织化学染色。Ⅰ型和Ⅲ型胶原染色的标本外观几乎相同。这些底物定位于骨髓造血区稀疏分布的一群细胞的细胞质和纤维状突起中，在骨髓血窦和脂肪间隙处尤为明显。因此，它们似乎与已知的网状细胞分布相似，尽管其形态在某些方面与后者的经典描述有所不同。Ⅳ型胶原与血窦的内皮衬里相关。其他结缔组织成分（骨、骨膜、骨内膜、血管等）表现出特征性的胶原异质性。这些结果表明，胶原是骨髓结缔组织的重要组成部分。
