苄青霉素制剂可在豚鼠中引发全身性过敏反应。

Benzylpenicillin preparations can evoke a systemic anaphylactic reaction in guinea pigs.

作者信息

Muranaka M, Suzuki S, Koizumi K, Igarashi H, Okumura H, Takeda K, Tadokoro K, Horiuchi Y

出版信息

J Allergy Clin Immunol. 1978 Nov;62(5):276-82. doi: 10.1016/0091-6749(78)90158-6.

Abstract

All of the five commercially available benzylpenicillin preparations obtained from different sources and a PcG preparation prepared by filtration of a commercial PcG on Sephadex G10 elicited the systemic anaphylactic reactions in guinea pigs which had been immunized with benzylpenicilloyl (BPO)-Ascaris extract conjugate (BPO-As) mixed with aluminum bydroxide gel. These preparations could evoke no such reactions in guinea pigs immunized with BPO-bovine gamma globulin conjugate (BPO-BGG) emulsified with complete Freund's adjuvant. The severity of the systemic anaphylactic reactions correlated significantly with the titers of either 8-day passive cutaneous anaphylactic (8-day PCA) reactions or 4-hr PCA reactions evoked with the same benzylpenicillin preparations. In vitro benzylpenicillin preparation contracted the tracheas of the guinea pigs immunized with BPO-As. These results indicated that the commercially available benzylpenicillin preparations have enough antigenicity to evoke systemic anaphylactic reactions in guinea pigs immunized with BPO-As mixed with aluminum hydroxide gel. Such guinea pigs represent an animal model for investigation of penicillin allergy.

摘要

从不同来源获得的所有五种市售苄青霉素制剂，以及通过在Sephadex G10上过滤市售青霉素G（PcG）制备的一种PcG制剂，在已用苄青霉素酰基（BPO）-蛔虫提取物偶联物（BPO-As）与氢氧化铝凝胶混合免疫的豚鼠中引发了全身性过敏反应。这些制剂在用完全弗氏佐剂乳化的BPO-牛γ球蛋白偶联物（BPO-BGG）免疫的豚鼠中不会引发此类反应。全身性过敏反应的严重程度与用相同苄青霉素制剂引发的8天被动皮肤过敏（8天PCA）反应或4小时PCA反应的滴度显著相关。体外苄青霉素制剂使用BPO-As免疫的豚鼠气管收缩。这些结果表明，市售苄青霉素制剂具有足够的抗原性，可在用氢氧化铝凝胶混合的BPO-As免疫的豚鼠中引发全身性过敏反应。此类豚鼠代表了用于研究青霉素过敏的动物模型。