Sheibani K, Tubbs R R, Gephardt G N, McMahon J T, Valenzuela R
Hum Pathol. 1981 Apr;12(4):349-54. doi: 10.1016/s0046-8177(81)80144-x.
Renal immunomicroscopy using enzyme labeled reagents has been shown to be a reliable method for the identification of immunoglobulins and complement in the routine evaluation of glomerular disease. However, the potential carcinogenicity of benzidine derivatives used in the procedure represents a major disadvantage of the technique. With a series of 55 renal biopsy specimens evaluated by light microscopy, immunofluorescence, and electron microscopy from patients with a variety of renal diseases, a study was done comparing aminoethylcarbazole and the Hanker-Yates reagent (p-phenylenediamine and pyrocatechol), chromogens chemically unrelated to benzidine. Aminoethylcarbazole was not suitable for renal immunomicroscopy, since in cases of antiglomerular basement membrane disease the color reaction product was finely granular at high magnification. Specimens immunostained with the Hanker-Yates reagent yielded permanent water insoluble reaction products and immunomicroscopic patterns identical to the results observed with immunofluorescence in all cases. To our knowledge, no carcinogenic properties have been identified for p-phenylenediamine or pyrocatechol. The Hanker-Yates reagent may be used routinely for renal enzyme immunomicroscopic studies with no currently identified carcinogenic hazard to laboratory personnel.
使用酶标记试剂的肾脏免疫显微镜检查已被证明是在肾小球疾病常规评估中鉴定免疫球蛋白和补体的可靠方法。然而，该过程中使用的联苯胺衍生物的潜在致癌性是该技术的一个主要缺点。对55例患有各种肾脏疾病的患者的肾活检标本进行了光学显微镜、免疫荧光和电子显微镜评估，开展了一项研究，比较氨基乙基咔唑和汉克-耶茨试剂（对苯二胺和邻苯二酚），这两种显色剂在化学上与联苯胺无关。氨基乙基咔唑不适用于肾脏免疫显微镜检查，因为在抗肾小球基底膜疾病的病例中，高倍镜下显色反应产物呈细颗粒状。用汉克-耶茨试剂免疫染色的标本产生了永久性的水不溶性反应产物，并且在所有病例中免疫显微镜模式与免疫荧光观察结果相同。据我们所知，尚未发现对苯二胺或邻苯二酚有致癌特性。汉克-耶茨试剂可常规用于肾脏酶免疫显微镜研究，目前未发现对实验室人员有致癌危害。
