Schaller K, Krauel A, Braun V
J Bacteriol. 1981 Jul;147(1):135-9. doi: 10.1128/jb.147.1.135-139.1981.
A mutant sensitive to colicin M at 30 degrees C and tolerant at 42 degrees C to high concentrations of colicin M was isolated from Escherichia coli K-12. A temperature shift from 30 to 42 degrees C rescued all cells up to the time they started to lyse at 30 degrees C (25 min after addition of colicin M). The growth rate at 42 degrees C remained unaffected by colicin M. AT 42 degrees C the cell-bound colicin M was inactivated by trypsin, sodium dodecyl sulfate, and antiserum against colicin M. Ferrichrome competed with colicin M at 42 degrees C only during the initial adsorption to the common receptor protein in the outer membrane. Since cells lysed earlier at 30 degrees C when they had been preincubated with colicin M at 42 degrees C, we conclude that the process leading finally to cell lysis is initiated at 42 degrees C and stops at a later stage of colicin M trypsin, dodecyl sulfate, and antiserum when cells were transferred from 30 to 42 degrees C, we assume that colicin M is translocated from its target site towards the cell surface. The mutation conferring tolerance was mapped close to the rpsL gene.
从大肠杆菌K-12中分离出一株在30℃对大肠菌素M敏感、在42℃对高浓度大肠菌素M耐受的突变体。从30℃到42℃的温度转变能挽救所有细胞，直到它们在30℃开始裂解（添加大肠菌素M后25分钟）。在42℃时生长速率不受大肠菌素M影响。在42℃时，细胞结合的大肠菌素M可被胰蛋白酶、十二烷基硫酸钠和抗大肠菌素M抗血清灭活。铁载体仅在42℃大肠菌素M最初吸附到外膜中的共同受体蛋白期间与之竞争。由于当细胞在42℃与大肠菌素M预孵育后在30℃更早裂解，我们得出结论，最终导致细胞裂解的过程在42℃启动，并在大肠菌素M被胰蛋白酶、十二烷基硫酸钠和抗血清作用的后期阶段停止。当细胞从30℃转移到42℃时，我们假设大肠菌素M从其靶位点向细胞表面转运。赋予耐受性的突变被定位在靠近rpsL基因的位置。
