Lavoie M, Mathieu L G
Can J Microbiol. 1975 Oct;21(10):1595-601. doi: 10.1139/m75-233.
An Escherichia coli K12 mutant resistant to colicin A-CA31 apparently through loss of its receptor sites has been isolated and partially characterized. Resistance to colicin A was accompanied with a decreased sensitivity to colicins L-398 and E2-CA42, and to acridine dyes. The mutant strain displayed the same general pattern of tolerance or sensitivity as the parent strain towards eight antibiotics, colicins C, D, E1, E3, F2, F3, G, I, K, and N; phages T1, T2, T5, T6, T7, F2, lambda vir, P1kc, phi 80, and BF23; and to methylene blue, triphenyltetrazolium chloride, ethylenediaminetetraacetate (EDTA), deoxycholate, and sodium dodecyl sulfate. Conjugation and transduction experiments showed that a locus controlling resistance to colicin A-CA31 mapped at 21 min on the genetic map of this E. coli K12 strain.
已分离出一株对大肠菌素A-CA31具有抗性的大肠杆菌K12突变体，其抗性显然是由于受体位点缺失所致，并对其进行了部分特性分析。对大肠菌素A的抗性伴随着对大肠菌素L-398和E2-CA42以及吖啶染料敏感性的降低。该突变菌株对八种抗生素（大肠菌素C、D、E1、E3、F2、F3、G、I、K和N）、噬菌体T1、T2、T5、T6、T7、F2、λvir、P1kc、φ80和BF23以及亚甲蓝、氯化三苯基四氮唑、乙二胺四乙酸（EDTA）、脱氧胆酸盐和十二烷基硫酸钠的耐受或敏感模式与亲本菌株相同。接合和转导实验表明，控制对大肠菌素A-CA31抗性的一个基因座位于该大肠杆菌K12菌株遗传图谱的21分钟处。
