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从水中分离堪萨斯分枝杆菌的改良技术。

Improved technique for isolation of Mycobacterium kansasii from water.

作者信息

Powell B L, Steadham J E

出版信息

J Clin Microbiol. 1981 May;13(5):969-75. doi: 10.1128/jcm.13.5.969-975.1981.

DOI:10.1128/jcm.13.5.969-975.1981
PMID:7016916
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC273925/
Abstract

A new concentration procedure, together with a new isolation medium, resulted in a 10- to 100-fold increase in the possibility of identifying Mycobacterium kansasii from water samples in comparison to a previously used procedure. In a survey which included both potable and natural water samples from many sites within the state of Texas, nine isolations of high-catalase strains of M. kansasii were obtained from 232 water samples tested. Acid-fast smear results were compared with mycobacterial isolations. An isolate from a river in central Texas is the first high-catalase strain of M. kansasii encountered in a natural water supply. Surveys of water samples from two Texas towns indicate that chlorine levels may influence the number of recoverable mycobacteria in water supplies.

摘要

一种新的浓缩方法,连同一种新的分离培养基,与先前使用的方法相比,使从水样中鉴定堪萨斯分枝杆菌的可能性提高了10至100倍。在一项涵盖得克萨斯州多个地点的饮用水和天然水样的调查中,从232份测试水样中获得了9株堪萨斯分枝杆菌的高过氧化氢酶菌株。将抗酸涂片结果与分枝杆菌分离结果进行了比较。从得克萨斯州中部一条河流中分离出的菌株是在天然水源中首次遇到的堪萨斯分枝杆菌高过氧化氢酶菌株。对得克萨斯州两个城镇的水样进行的调查表明,氯含量可能会影响供水系统中可回收分枝杆菌的数量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d139/273925/79af857a83a6/jcm00166-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d139/273925/79af857a83a6/jcm00166-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d139/273925/79af857a83a6/jcm00166-0189-a.jpg

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Differentiation of mycobacterial species by PCR-restriction analysis of DNA (342 base pairs) of the RNA polymerase gene (rpoB).通过对RNA聚合酶基因(rpoB)的DNA(342个碱基对)进行PCR限制性分析来鉴别分枝杆菌菌种。
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本文引用的文献

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A METHOD FOR THE ISOLATION OF UNCLASSIFIED ACID-FAST BACILLI FROM SOIL AND WATER.一种从土壤和水中分离未分类抗酸杆菌的方法。
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High-catalase strains of Mycobacterium kansasii isolated from water in Texas.从得克萨斯州的水中分离出的堪萨斯分枝杆菌的高过氧化氢酶菌株。
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The isolation of high catalase Mycobacterium kansasii from tap water.从自来水中分离出高过氧化氢酶堪萨斯分枝杆菌。
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