Hayashi M
Nihon Sanka Fujinka Gakkai Zasshi. 1980 Jan;32(1):94-102.
The enzyme-labeled antibody method was used to examine the localized distribution and variation by gestation week of HCG and HPL in placenta of 44 cases. 1) HCG: Electron microscopy revealed HRP reaction products in cisterna and membrane of perinuclear spaces and endoplasmic reticulum of syncytial cell (S-cell), but not in surface of S-cell microvilli or in Langhans' cell (L-cell). HRP reaction products in S-cell were more appreciable during 5th-12th weeks of gestation in syncytial sprouts, junction of villi with decidua, and rami or ramuli chorii, and fewer after 12th weeks. 2) HPL: Electron microscopy revealed reaction products in nuclear membrane and membrane of endoplasmic reticulum and Golgi complex, but not in surface of S-cell microvilli or L-cell. Reaction products in S-cell were noted from 8th gestation week, and most appreciably in 16th-18th gestation weeks preceding peak HPL concentration in serum. This indicates HPL exists in S-cell in the from of Pre. Pro-HPL.
采用酶标抗体法对44例胎盘绒毛膜促性腺激素（HCG）和人胎盘催乳素（HPL）按孕周进行定位分布及变化观察。1）HCG：电镜观察，合体细胞（S细胞）核周间隙及内质网池和膜上有辣根过氧化物酶（HRP）反应产物，而S细胞微绒毛表面及郎罕细胞（L细胞）内无。S细胞内的HRP反应产物在妊娠5～12周时于合体细胞芽、绒毛与蜕膜交界处及绒毛分支处较明显，12周后减少。2）HPL：电镜观察，核膜、内质网膜及高尔基复合体内有反应产物，S细胞微绒毛表面及L细胞内无。S细胞内的反应产物自妊娠8周开始出现，于血清HPL浓度高峰前的妊娠16～18周时最为明显。这表明HPL在S细胞内以Pre.Pro - HPL形式存在。
