Morrish D W, Marusyk H
Perinatal Research Centre, Signal Transduction Laboratories, University of Alberta and W.W. Cross Cancer Institute, Edmonton, Canada.
Microsc Res Tech. 1997;38(1-2):176-87. doi: 10.1002/(SICI)1097-0029(19970701/15)38:1/2<176::AID-JEMT18>3.0.CO;2-M.
To demonstrate human chorionic gonadotropin (hCG) and human placental lactogen (hPL) secretory granules in placenta and to illustrate newer embedding techniques and specific immunospecificity problems in the placenta, labeling experiments using immunogold or peroxidase combined with avidin-biotin enhancement in epoxy LX-112-, Araldite-, or LR gold-embedded tissue fixed in 2.5% glutaraldehyde or 2.5% paraformaldehyde were carried out in term and first-trimester normal human placenta and in partial hydatidiform moles. Increased sensitivity of the low-temperature LR gold method was found for hPL-labeled granules. beta hCG-labeled granules were noted in syncytium of first-trimester placenta, and beta hCG-containing granules in hydatidiform moles were similar to those of normal placenta. Paraformaldehyde fixation and LR gold embedding permitted identification of endoplasmic reticulum-associated labeling not observed with other methods. A brief review and discussion of immunolabeling methods, controls, and embedding materials is presented. We conclude that further refinement of peptide localization methods in the placenta is possible but must take into account the abundant potentially cross-reacting peptides present in the placenta.
为了在胎盘中显示人绒毛膜促性腺激素（hCG）和人胎盘催乳素（hPL）分泌颗粒，并说明胎盘中更新的包埋技术和特异性免疫特异性问题，我们在足月和孕早期正常人类胎盘以及部分葡萄胎中，对用免疫金或过氧化物酶结合抗生物素蛋白-生物素增强技术处理的、固定于2.5%戊二醛或2.5%多聚甲醛中的环氧LX-112、环氧树脂或LR金包埋组织进行了标记实验。发现低温LR金方法对hPL标记颗粒的敏感性增加。在孕早期胎盘的合体滋养层中观察到β-hCG标记颗粒，葡萄胎中含β-hCG的颗粒与正常胎盘的相似。多聚甲醛固定和LR金包埋能够识别其他方法未观察到的内质网相关标记。本文对免疫标记方法、对照和包埋材料进行了简要回顾和讨论。我们得出结论，虽然胎盘肽定位方法有可能进一步完善，但必须考虑到胎盘中存在大量可能发生交叉反应的肽。
