Brade L, Müller-Lantzsch N, zur Hausen H
J Med Virol. 1981;6(4):301-8. doi: 10.1002/jmv.1890060405.
A novel member of the papovavirus group has been isolated from EBV-transformed African green monkey (AGM) B-lymphoblasts. The virus is characterized by its B-lymphotropic host range and has tentatively been named "lymphotropic papovavirus" (LPV). Seroepidemiological studies revealed that besides sera from African green monkeys a substantial proportion of human sera reacted with antigens of this virus. The specificity of this reaction observed in indirect immunofluorescence tests was underlined by immunoprecipitation (IP) and neutralization studies. By IP we demonstrated that AGM as well as human sera reacting in immunofluorescence with LPV antigens precipitated polypeptides of about 40 K. Those polypeptides were detected only in LPV-infected cultures. Sera of high reactivity in immunofluorescence tests also neutralized viral infectivity. These data suggest that an agent antigenetically closely related to AGM-LPV also infects part of the human population. The age distribution of the human antibody response revealed that a low percentage of sera reacted in age groups below 30 years. Thereafter, however, about 30% of all sera exhibited antibodies to LPV antigens. There was a slight increase in seroreactivity in sera from patients tested for infectious hepatitis when compared to non-selected human sera.
从爱泼斯坦-巴尔病毒（EBV）转化的非洲绿猴（AGM）B淋巴细胞中分离出一种乳头多瘤空泡病毒组的新型成员。该病毒以其嗜B淋巴细胞的宿主范围为特征，并暂定名为“嗜淋巴细胞乳头多瘤空泡病毒”（LPV）。血清流行病学研究表明，除了来自非洲绿猴的血清外，相当一部分人类血清也能与该病毒的抗原发生反应。免疫沉淀（IP）和中和研究进一步证实了在间接免疫荧光试验中观察到的这种反应的特异性。通过IP，我们证明在免疫荧光中与LPV抗原发生反应的AGM血清以及人类血清沉淀出了约40K的多肽。这些多肽仅在LPV感染的培养物中检测到。免疫荧光试验中高反应性的血清也能中和病毒感染性。这些数据表明，一种在抗原上与AGM-LPV密切相关的病原体也感染了一部分人群。人类抗体反应的年龄分布显示，30岁以下年龄组中只有一小部分血清发生反应。然而，此后，所有血清中约30%表现出针对LPV抗原的抗体。与未经过挑选的人类血清相比，检测传染性肝炎的患者血清中的血清反应性略有增加。
