Schoulaker R, Engelberg-Kulka H
J Virol. 1978 Jan;25(1):433-5. doi: 10.1128/JVI.25.1.433-435.1978.
The temperature-sensitive conjugational transfer-deficient mutant Escherichia coli JCFL39, carrying a traD(Ts) mutation, is herein described as also being temperature sensitive for group I RNA phages (MS2, f2, and R17) but not for Q beta. Temperature shift experiments showed that the growth of group I phage MS2 in the mutant could be inhibited by a post-penetration event at high temperature. A possible role for the traD cistron of sex factor F in the intracellular development of MS2 is suggested.
本文描述了携带traD(Ts)突变的温度敏感型接合转移缺陷型突变体大肠杆菌JCFL39,它对I组RNA噬菌体(MS2、f2和R17)也具有温度敏感性,但对Qβ不敏感。温度转换实验表明,高温下的穿透后事件可抑制I组噬菌体MS2在该突变体中的生长。这表明性因子F的traD顺反子在MS2的细胞内发育中可能发挥作用。