Orci L, Miller R G, Montesano R, Perrelet A, Amherdt M, Vassalli P
Science. 1980 Nov 28;210(4473):1019-21. doi: 10.1126/science.7434010.
Filipin binding to membrane sterols induces deformations of the membrane that are detected by freeze-fracture either as 20- to 25-nanometer protuberances or as pits on the fracture faces. By using the filipin probe in pancreatic acinar cells, it was found that the polarity of filipin-induced deformations in the membrane limiting the Golgi condensing vacuoles is opposite that in the membrane limiting the mature zymogen granules. This asymmetry could be due to unequal partitioning of cholesterol between the membrane leaflets in these two compartments during the transformation of the condensing vacuole into the zymogen granule.
菲律宾菌素与膜固醇结合会诱导膜变形，这种变形在冷冻蚀刻中可检测到，表现为20至25纳米的突起或蚀刻面上的凹坑。通过在胰腺腺泡细胞中使用菲律宾菌素探针，发现菲律宾菌素诱导的限制高尔基体浓缩泡的膜变形极性与限制成熟酶原颗粒的膜变形极性相反。这种不对称可能是由于在浓缩泡转化为酶原颗粒的过程中，这两个区室的膜小叶之间胆固醇分配不均所致。
