Jost P C, Griffith O H
Pharmacol Biochem Behav. 1980;13 Suppl 1:155-65. doi: 10.1016/s0091-3057(80)80025-6.
Over the past decade spectroscopic methods (fluorescence, ESR, and NMR) have been used to provide new information about the molecular dynamics of lipid-lipid and lipid-protein interactions in membranes. The various methods of characterizing isotropic and anisotropic motion are described. Lipid bilayers are highly dynamic, with rapid acyl chain motion and rapid lateral diffusion in the plane of the membrane. In membranes where proteins penetrate through the bilayer, a large hydrophobic surface area exists in contact with the bilayer lipids. Lipids at the protein interface are in dynamic equilibrium with the remaining pools of bilayer. The protein has been shown spectroscopically to have some influence on the dynamics of the nearest neighbor lipids, leaving the rest of the bilayer relatively unperturbed. Evidence is summarized that, in some cases, the lipid composition in the interfacial region is influenced by the protein.
在过去十年中，光谱方法（荧光、电子自旋共振和核磁共振）已被用于提供有关膜中脂质 - 脂质和脂质 - 蛋白质相互作用分子动力学的新信息。描述了表征各向同性和各向异性运动的各种方法。脂质双层具有高度动态性，酰基链运动迅速，且在膜平面内横向扩散迅速。在蛋白质穿透双层的膜中，存在与双层脂质接触的大疏水表面积。蛋白质界面处的脂质与双层其余部分的脂质处于动态平衡。通过光谱学已证明蛋白质对最近邻脂质的动力学有一定影响，而双层的其余部分相对未受干扰。总结的证据表明，在某些情况下，界面区域的脂质组成受蛋白质影响。
