细胞与发育生物学中的激光显微手术

Laser microsurgery in cell and developmental biology.

作者信息

Berns M W, Aist J, Edwards J, Strahs K, Girton J, McNeill P, Rattner J B, Kitzes M, Hammer-Wilson M, Liaw L H, Siemens A, Koonce M, Peterson S, Brenner S, Burt J, Walter R, Bryant P J, van Dyk D, Coulombe J, Cahill T, Berns G S

出版信息

Science. 1981 Jul 31;213(4507):505-13. doi: 10.1126/science.7017933.

DOI:10.1126/science.7017933

PMID:7017933

Abstract

New applications of laser microbeam irradiation to cell and developmental biology include a new instrument with a tunable wavelength (217- to 800-nanometer) laser microbeam and a wide range of energies and exposure durations (down to 25 X 10(-12) second). Laser microbeams can be used for microirradiation of selected nucleolar genetic regions and for laser microdissection of mitotic and cytoplasmic organelles. They are also used to disrupt the developing neurosensory appendages of the cricket and the imaginal discs of Drosophila.

摘要

激光微束辐照在细胞与发育生物学中的新应用包括一种新型仪器，它配备了波长可调（217至800纳米）的激光微束，以及广泛的能量范围和曝光持续时间（低至25×10⁻¹²秒）。激光微束可用于对选定的核仁遗传区域进行微辐照，以及对有丝分裂和细胞质细胞器进行激光显微切割。它们还被用于破坏蟋蟀发育中的神经感觉附属器官以及果蝇的成虫盘。