Martin E D, Rault A, Brunaud M D, Guery C
Ann Pathol. 1981;1(1):77-85.
Three recent progresses allow to perform plastic embedding of moderate or large samples of tissue in limits of time close to those of the paraffin embedding and to obtain slices between 1 and 2,5 mu on which all current histochemical or even histo-immunological reactions are possible. These technics prevent the retraction of the tissues, give a good preservation of the structure of the cells and permit a good analysis of the tiny lesions of all kinds of tissues. From our experience about 1362 varied biopsies, the best indications seems to be in order : kidney, hepatic, lung, medullary and nodes biopsies. It seems now justified to introduce these technics as a routine one, inside laboratories which have to detect tiny lesions on particular tissues or on experimental samples.
最近的三项进展使得能够在接近石蜡包埋的时间限制内对中等或大尺寸的组织样本进行塑料包埋,并获得1至2.5微米的切片,在这些切片上可以进行所有当前的组织化学甚至组织免疫反应。这些技术可防止组织收缩,能很好地保存细胞结构,并有助于对各种组织的微小病变进行良好分析。根据我们对1362份不同活检样本的经验,最佳适应证似乎依次为:肾脏、肝脏、肺、髓质和淋巴结活检。现在,在必须检测特定组织或实验样本上微小病变的实验室中,将这些技术作为常规技术引入似乎是合理的。