体外染色质转录的特异性。大肠杆菌RNA聚合酶对珠蛋白基因的不对称转录。

Specificity of chromatin transcription in vitro. Asymmetric transcription of the globin gene by Escherichia coli RNA polymerase.

作者信息

Pays E, Gilmour R S

出版信息

Biochim Biophys Acta. 1981 May 29;653(3):356-67. doi: 10.1016/0005-2787(81)90192-1.

DOI:10.1016/0005-2787(81)90192-1

Abstract

The transcription of globin genes in mouse foetal liver chromatin and nuclei by exogenous Escherichia coli RNA polymerase is prone to artifacts due to RNA-dependent transcription of endogenous mRNA sequences. This is particularly evident when Mn2+ is used as divalent cation in the RNA transcription reaction. However, substitution or supplementation with Mg2+ eliminates this artifact and gives essentially asymmetric DNA-dependent transcription by the polymerase. In this paper we discuss a number of general criteria which can be applied to test the validity of specific gene transcription in vitro.

摘要

在外源大肠杆菌RNA聚合酶作用下，小鼠胎儿肝脏染色质和细胞核中珠蛋白基因的转录容易出现假象，这是由于内源性mRNA序列的RNA依赖性转录所致。当在RNA转录反应中使用Mn2+作为二价阳离子时，这种情况尤为明显。然而，用Mg2+替代或补充则可消除这种假象，并使聚合酶基本上进行不对称的DNA依赖性转录。在本文中，我们讨论了一些可用于检验体外特定基因转录有效性的通用标准。

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