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人类ε珠蛋白基因的染色质结构：核酸酶超敏位点与多个转录起始位点相关。

The chromatin structure of the human epsilon globin gene: nuclease hypersensitive sites correlate with multiple initiation sites of transcription.

作者信息

Zhu J, Allan M, Paul J

出版信息

Nucleic Acids Res. 1984 Dec 11;12(23):9191-204. doi: 10.1093/nar/12.23.9191.

DOI:10.1093/nar/12.23.9191
PMID:6096822
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320448/
Abstract

We have mapped sites in chromatin flanking the epsilon-globin gene in the K562 cell which are hypersensitive to digestion with DNAseI, micrococcal nuclease and S1 nuclease. Many of those in the 5' flanking region correspond to minor upstream transcriptional starts. However, one prominent site occurs upstream of the boundary of transcription; it maps to a region with an unusual DNA sequence. In baby hamster kidney cells stably transformed with recombinant DNA containing the human epsilon-globin gene and in Cos 7 cells transiently transfected with DNA containing the epsilon-globin gene, hypersensitive sites can be demonstrated.

摘要

我们已经绘制了K562细胞中ε-珠蛋白基因侧翼染色质上对DNA酶I、微球菌核酸酶和S1核酸酶消化敏感的位点。5'侧翼区域的许多位点对应于次要的上游转录起始位点。然而，一个显著的位点出现在转录边界的上游；它定位于一个具有异常DNA序列的区域。在用含有人ε-珠蛋白基因的重组DNA稳定转化的幼仓鼠肾细胞以及用含ε-珠蛋白基因的DNA瞬时转染的Cos 7细胞中，可以证明存在超敏位点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b103/320448/66f0ce1d4a09/nar00341-0449-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b103/320448/9a3e9e138601/nar00341-0443-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b103/320448/900d0f8b1dfb/nar00341-0445-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b103/320448/f235dce45760/nar00341-0447-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b103/320448/354bc7f0878d/nar00341-0448-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b103/320448/66f0ce1d4a09/nar00341-0449-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b103/320448/9a3e9e138601/nar00341-0443-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b103/320448/900d0f8b1dfb/nar00341-0445-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b103/320448/f235dce45760/nar00341-0447-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b103/320448/354bc7f0878d/nar00341-0448-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b103/320448/66f0ce1d4a09/nar00341-0449-a.jpg

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1
The chromatin structure of the human epsilon globin gene: nuclease hypersensitive sites correlate with multiple initiation sites of transcription.人类ε珠蛋白基因的染色质结构：核酸酶超敏位点与多个转录起始位点相关。
Nucleic Acids Res. 1984 Dec 11;12(23):9191-204. doi: 10.1093/nar/12.23.9191.
2
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J Biol Chem. 1982 Jul 10;257(13):7730-6.
5
Mapping of DNase I-hypersensitive sites in the upstream DNA of human embryonic epsilon-globin gene in K562 leukemia cells.K562白血病细胞中人胚胎ε-珠蛋白基因上游DNA中DNase I超敏位点的定位
Proc Natl Acad Sci U S A. 1984 May;81(9):2718-22. doi: 10.1073/pnas.81.9.2718.
6
Distant sequences which regulate globin genes.调控珠蛋白基因的远距序列。
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Erythroid-specific nuclease-hypersensitive sites flanking the human beta-globin domain.人类β-珠蛋白结构域两侧的红系特异性核酸酶超敏位点。
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An altered DNA conformation detected by S1 nuclease occurs at specific regions in active chick globin chromatin.通过S1核酸酶检测到的DNA构象改变发生在活跃的鸡珠蛋白染色质的特定区域。
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Accurate initiation of human epsilon-globin RNA synthesis by Escherichia coli RNA polymerase in isolated nuclei of K562 erythroleukemia cells.大肠杆菌RNA聚合酶在K562红白血病细胞分离细胞核中准确起始人ε-珠蛋白RNA合成。
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A developmentally stable chromatin structure in the human beta-globin gene cluster.人类β-珠蛋白基因簇中一种发育稳定的染色质结构。
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1359-63. doi: 10.1073/pnas.83.5.1359.

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Mol Cell Biol. 1996 Nov;16(11):6055-64. doi: 10.1128/MCB.16.11.6055.
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本文引用的文献

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The inheritance of methylation patterns in vertebrates.脊椎动物中甲基化模式的遗传
Cell. 1981 May;24(2):285-6. doi: 10.1016/0092-8674(81)90317-2.
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Specificity of chromatin transcription in vitro. Asymmetric transcription of the globin gene by Escherichia coli RNA polymerase.体外染色质转录的特异性。大肠杆菌RNA聚合酶对珠蛋白基因的不对称转录。
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Major changes in the 5' and 3' chromatin structure of sea urchin histone genes accompany their activation and inactivation in development.
红细胞生成过程中α1和β珠蛋白主基因以及谷胱甘肽过氧化物酶基因的次要转录本变化。
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A developmentally stable chromatin structure in the human beta-globin gene cluster.人类β-珠蛋白基因簇中一种发育稳定的染色质结构。
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1359-63. doi: 10.1073/pnas.83.5.1359.
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Organization, sequence and nuclease hypersensitivity of repetitive elements flanking the chicken apoVLDLII gene: extended sequence similarity to elements flanking the chicken vitellogenin gene.鸡载脂蛋白VLDLII基因侧翼重复元件的组织、序列及核酸酶超敏感性：与鸡卵黄蛋白原基因侧翼元件的序列相似性扩展
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Tissue specific transcription of the human epsilon-globin gene following transfection into the embryonic erythroid cell line K562.将人ε-珠蛋白基因转染至胚胎红细胞系K562后该基因的组织特异性转录。
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RNA processing of beta-globin transcripts containing 5' flanking and structural gene sequences.包含5'侧翼和结构基因序列的β-珠蛋白转录本的RNA加工
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Identification of a transcriptional silencer in the 5'-flanking region of the human epsilon-globin gene.人类ε-珠蛋白基因5'侧翼区转录沉默子的鉴定。
Proc Natl Acad Sci U S A. 1989 Jul;86(14):5306-9. doi: 10.1073/pnas.86.14.5306.
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Binding of protein uH2A and histone H2A to DNA.蛋白质uH2A和组蛋白H2A与DNA的结合。
Cell Biophys. 1989 Feb;14(1):43-51. doi: 10.1007/BF02797390.
10
Negative regulation of the human epsilon-globin gene by transcriptional interference: role of an Alu repetitive element.转录干扰对人ε-珠蛋白基因的负调控：Alu重复元件的作用
Mol Cell Biol. 1990 Mar;10(3):1209-16. doi: 10.1128/mcb.10.3.1209-1216.1990.
海胆组蛋白基因5'和3'染色质结构的重大变化伴随着它们在发育过程中的激活和失活。
Cell. 1983 Jul;33(3):843-8. doi: 10.1016/0092-8674(83)90026-0.
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The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.染色质中果蝇热休克基因的5'端对脱氧核糖核酸酶I高度敏感。
Nature. 1980 Aug 28;286(5776):854-60. doi: 10.1038/286854a0.
5
Alternative sites of transcription initiation upstream of the canonical cap site in human gamma-globin and beta-globin genes.人类γ-珠蛋白基因和β-珠蛋白基因中位于典型帽位点上游的转录起始替代位点。
Nucleic Acids Res. 1984 Feb 24;12(4):1811-20. doi: 10.1093/nar/12.4.1811.
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DNA sequence changes in an upstream DNase I-hypersensitive region are correlated with reduced gene expression.上游DNA酶I超敏反应区域的DNA序列变化与基因表达降低相关。
Proc Natl Acad Sci U S A. 1983 Feb;80(4):1063-7. doi: 10.1073/pnas.80.4.1063.
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The unmethylated state of the promoter/leader and 5'-regions of integrated adenovirus genes correlates with gene expression.整合腺病毒基因的启动子/前导序列和5'区域的未甲基化状态与基因表达相关。
EMBO J. 1982;1(4):409-14. doi: 10.1002/j.1460-2075.1982.tb01183.x.
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Transfer of human globin genes to erythroleukemic mouse cells.将人类珠蛋白基因转移至红白血病小鼠细胞。
EMBO J. 1982;1(1):15-20. doi: 10.1002/j.1460-2075.1982.tb01117.x.
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Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult beta-globin gene.赋予鸡成年β-珠蛋白基因5'端核酸酶超敏感性的特定因子。
Proc Natl Acad Sci U S A. 1984 Jan;81(1):95-9. doi: 10.1073/pnas.81.1.95.
10
DNA methylation and gene activity.DNA甲基化与基因活性。
Annu Rev Biochem. 1983;52:93-124. doi: 10.1146/annurev.bi.52.070183.000521.