磷脂囊泡中磷脂酰乙醇胺的质子交换动力学
Kinetics of proton exchange of phosphatidylethanolamine in phospholipid vesicles.
作者信息
Ralph E K, Lange Y, Redfield A G
出版信息
Biophys J. 1985 Dec;48(6):1053-7. doi: 10.1016/S0006-3495(85)83868-6.
Abstract
The rate of proton exchange of the amino protons of phosphatidylethanolamine (PE) in sonicated mixed phospholipid vesicles has been determined by NMR spectroscopy. The rate of exchange increases with increasing pH and phosphate concentration. In the absence of buffer the dominant exchange process is an intrasurface reaction in which NH2 groups react via water with NH3+ groups on the outer surface. Addition of cholesterol reduces the rate constant for intrasurface exchange. The experiments are evidence that such reactions could be dominant in proton transport in and to membrane surfaces.
摘要
通过核磁共振光谱法测定了超声处理的混合磷脂囊泡中磷脂酰乙醇胺（PE）氨基质子的质子交换速率。交换速率随pH值和磷酸盐浓度的增加而增加。在没有缓冲剂的情况下，主要的交换过程是表面内反应，其中NH2基团通过水与外表面的NH3+基团反应。添加胆固醇会降低表面内交换的速率常数。这些实验证明，此类反应在质子向膜表面的运输及膜表面内的质子运输中可能起主导作用。
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