Chopra D P, Yeh K Y
In Vitro. 1981 May;17(5):441-9. doi: 10.1007/BF02626745.
Serial passage cultures of colonic epithelial cells from young rats have been maintained for more than 6 months in Eagle's minimum essential medium buffered with HEPES (25 mM) and supplemented with 2.5% fetal bovine serum, 0.5 micrograms/ml insulin, 5.0 micrograms/ml transferrin, and antibiotics. The cells proliferated in this medium with a population doubling time of approximately 53 h. The cells retained differentiated morphology as evidenced by secretory activity and the presence of secretory granules, microvilli, tonofilaments, and desmosomal junctions. Further, cells at the fourth passage had normal karyotypes with 42 chromosomes and exhibited anchorage dependent growth. High concentrations of fetal bovine serum (10 to 15%) exerted toxic effects on the colonic epithelial cell cultures.
来自幼鼠的结肠上皮细胞连续传代培养物已在含有HEPES（25 mM）缓冲的伊格尔最低必需培养基中维持了6个月以上，并补充有2.5%胎牛血清、0.5微克/毫升胰岛素、5.0微克/毫升转铁蛋白和抗生素。细胞在这种培养基中增殖，群体倍增时间约为53小时。细胞保留了分化形态，分泌活性以及分泌颗粒、微绒毛、张力丝和桥粒连接的存在证明了这一点。此外，第四代细胞具有42条染色体的正常核型，并表现出贴壁依赖性生长。高浓度的胎牛血清（10%至15%）对结肠上皮细胞培养物产生毒性作用。
