Fonti R, Latella G, Bises G, Magliocca F, Nobili F, Caprilli R, Sambuy Y
Cattedra di Gastroenterologia, Università de L'Aquila, Italy.
Int J Colorectal Dis. 1994 Apr;9(1):13-22. doi: 10.1007/BF00304294.
The purpose of this work was to set up an in vitro model for the study of normal and pathological functions of the colonic epithelium. We have isolated colonic crypts by mild proteolytic digestion and mechanical dissociation of human biopsy material obtained during colonoscopy. The crypts, free of connective tissue, when placed in culture rapidly attached to the substrate and formed colonies containing over 95% of epithelial cells. Histochemical and ultrastructural characterization of the colonies showed the presence of both absorptive and secretory cells, exhibiting a high degree of differentiation. Proliferative activity occurred mostly during the first 24 h and progressively declined thereafter. The cells survived and maintained differentiated characteristics for at least three days in culture. This method can be used to study normal functions of the colonic epithelium. It may also be employed to investigate both noxious and protective factors in pathological conditions such as inflammatory bowel disease and colorectal neoplasia.
这项工作的目的是建立一个体外模型，用于研究结肠上皮的正常和病理功能。我们通过对结肠镜检查期间获取的人类活检材料进行温和的蛋白水解消化和机械解离，分离出了结肠隐窝。这些不含结缔组织的隐窝在培养时迅速附着于基质并形成了包含超过95%上皮细胞的集落。集落的组织化学和超微结构特征显示存在吸收细胞和分泌细胞，表现出高度的分化。增殖活性主要发生在最初的24小时内，此后逐渐下降。细胞在培养中存活并保持分化特征至少三天。这种方法可用于研究结肠上皮的正常功能。它也可用于研究诸如炎症性肠病和结直肠癌等病理状况中的有害和保护因素。
