一种测定血浆中去甲变肾上腺素的放射酶法。
A radioenzymatic method for determination of normetanephrine in blood plasma.
作者信息
Nagel-Hiemke M, Schümann H J
出版信息
J Biochem Biophys Methods. 1981 Jun;4(5-6):255-9. doi: 10.1016/0165-022x(81)90065-8.
A radioenzymatic assay for determination of normetanephrine in blood plasma is described. It was based on N-methylation of normetanephrine by phenylethanolamine-N-methyltransferase using S-adenosyl[methyl-3]methionine as the methyl donor. 2981 +/- 85 cpm (mean +/- s mean, n = 32) were obtained from 1 ng normetanephrine. Blank values corresponded to 27 +/- 3 pg (n = 7). Normetanephrine levels measured in the blood plasma of 12 persons ranged from 1.3 to 9.6 ng/ml.
本文描述了一种用于测定血浆中去甲变肾上腺素的放射酶分析法。该方法基于苯乙醇胺-N-甲基转移酶以S-腺苷基[甲基-3]甲硫氨酸作为甲基供体对去甲变肾上腺素进行N-甲基化反应。1 ng去甲变肾上腺素可产生2981±85 cpm（平均值±标准误，n = 32）。空白值相当于27±3 pg（n = 7）。对12名受试者血浆中去甲变肾上腺素水平的测定结果为1.3至9.6 ng/ml。
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