Danley D L, Hilger A E
J Immunol. 1981 Aug;127(2):551-6.
Murine polymorphonuclear leukocytes (PMN) are stimulated to produce hydrogen peroxide (H2O2) by some unopsonized fungi, including Coccidioides immitis spherules, Candida albicans blastospores, and Saccharomyces sp. blastospores (zymosan). In this communication we have examined the basis for this stimulation by studying the effects of mannan and its primary constituent, D-mannose, on PMN function in vitro, since this polysaccharide is a significant component in many fungal cell walls. Our results show that mannan stimulated H2O2 production and iodination by PMN and enhanced H2O2 production by cells stimulated with zymosan. Moreover, the amount of H2O2 released by PMN was directly related to the concentration of mannan, and mannan obtained commercially or prepared in our laboratory worked equally well. Mannose, on the other hand, inhibited H2O2 release from PMN stimulated with zymosan or mannan and reduced oxygen consumption, carbon dioxide production, superoxide release, and iodination by these cells as well. With respect to specificity of inhibition, 18 different monosaccharides were examined by using 2 different assays for H2O2 release; and only 2-deoxy-D-glucose, a potent glycolytic inhibitor, reduced H2O2 release from zymosan-stimulated PMN. Furthermore, H2O2 release from cells stimulated with phorbol myristate acetate or opsonized Sephadex was not inhibited by mannose whereas H2O2 release was reduced when PMN were stimulated with C. albicans blastospores or C. immitis spherules in the presence of this sugar. From these data we propose that initiation of PMN oxidative metabolic burst in response to some unopsonized fungi occurs through a mannose-specific mechanism.
一些未被调理的真菌，包括粗球孢子菌小球体、白色念珠菌芽生孢子和酿酒酵母芽生孢子（酵母聚糖），可刺激小鼠多形核白细胞（PMN）产生过氧化氢（H2O2）。在本报告中，我们通过研究甘露聚糖及其主要成分D-甘露糖对体外PMN功能的影响，来探究这种刺激作用的基础，因为这种多糖是许多真菌细胞壁的重要组成部分。我们的结果表明，甘露聚糖可刺激PMN产生H2O2和进行碘化反应，并增强酵母聚糖刺激的细胞产生H2O2的能力。此外，PMN释放的H2O2量与甘露聚糖的浓度直接相关，市售或我们实验室制备的甘露聚糖效果相同。另一方面，甘露糖可抑制酵母聚糖或甘露聚糖刺激的PMN释放H2O2，并降低这些细胞的氧消耗、二氧化碳产生、超氧化物释放和碘化反应。关于抑制的特异性，我们使用2种不同的H2O2释放测定方法检测了18种不同的单糖；只有2-脱氧-D-葡萄糖，一种有效的糖酵解抑制剂，可减少酵母聚糖刺激的PMN释放H2O2。此外，佛波醇肉豆蔻酸酯或调理的葡聚糖刺激的细胞释放H2O2不受甘露糖抑制，而在这种糖存在的情况下，白色念珠菌芽生孢子或粗球孢子菌小球体刺激PMN时，H2O2释放会减少。根据这些数据，我们提出，PMN对某些未被调理的真菌产生氧化代谢爆发是通过一种甘露糖特异性机制启动的。
