Marcano C
Mycopathologia. 1981 Jun 5;74(3):135-41. doi: 10.1007/BF00437155.
Two hundred and twenty nine patients were sorted out at random from different services at the Hospital Universitario de Caracas, Venezuela, to investigate simultaneously the presence of Candida albicans in the mouth and in their toothbrushes. None of them showed signs of candidiasis. From the oral cavity of each patient two samples were taken by rubbing throughout the mucous membrane. One of them was cultured in oxgall agar and the other one in diluted milk agar. At the same time, the toothbrushes of the patients were cultured in oxgall agar or in diluted milk agar. The results showed evidence of C. albicans in the mouth of 28% of the patients hospitalized for different reasons, and in the 18% of the toothbrushes studied. Both media were found equally efficient for the diagnosis and identification of C. albicans in nascent culture, even with material contaminated with other yeasts and bacteria. The use of dental prosthesis, in patients without teeth and those with a few teeth, appears to be a predisposing factor for the colonization by C. albicans in healthy mouths. Out of 57 healthy mouth-carriers of C. albicans, 33 (58% of them ) had also C. albicans in their toothbrushes. These findings seem to point to the toothbrushes as being the possible reservoir and reinfection source in some cases of candidiasis. To patients suffering with oral candidiasis the use of some method for disinfecting the toothbrushes must be recommended when planning the treatment.
从委内瑞拉加拉加斯大学医院的不同科室随机挑选出229名患者，以同时调查白色念珠菌在其口腔和牙刷中的存在情况。他们均无念珠菌病迹象。从每位患者的口腔中通过擦拭整个黏膜采集两份样本。其中一份在牛胆汁琼脂中培养，另一份在稀释牛奶琼脂中培养。与此同时，患者的牙刷也在牛胆汁琼脂或稀释牛奶琼脂中培养。结果显示，因不同原因住院的患者中，28%的患者口腔中有白色念珠菌，在研究的牙刷中有18%检测到白色念珠菌。两种培养基在新生培养物中对白色念珠菌的诊断和鉴定同样有效，即使材料被其他酵母和细菌污染。对于无牙和牙齿较少的患者，使用假牙似乎是白色念珠菌在健康口腔中定植的一个易感因素。在57名口腔携带白色念珠菌的健康人中，有33人（占58%）的牙刷中也有白色念珠菌。这些发现似乎表明，在某些念珠菌病病例中，牙刷可能是储存库和再感染源。对于患有口腔念珠菌病的患者，在制定治疗方案时，必须建议使用某种方法对牙刷进行消毒。
