The Key Laboratory of Oral Biomedicine, Department of Conservative Dentistry and Endodontics, The Affiliated Stomatological Hospital of Nanchang University, Nanchang, China.
School of Stomatology, Nanchang University, Nanchang, China.
Front Cell Infect Microbiol. 2020 Feb 4;10:17. doi: 10.3389/fcimb.2020.00017. eCollection 2020.
Tooth brushing is necessary to maintain oral health. Little research has been carried out to explore microbial diversity in toothbrushes and to study the potential impact of these bacteria on human health. In the present study, 20 participants were enrolled, and the microbial diversity in their oral cavity and toothbrushes was investigated using high-throughput sequencing. Our results indicate that 1,136 and 976 operational taxonomic units (OTUs) were obtained from groups CB (samples from toothbrushes of participants using traditional Chinese medicinal toothpaste) and AB (samples from toothbrushes of those using antibacterial toothpaste), respectively. The pathogens , and were identified on toothbrushes. The presence of these pathogens increases the chance for the host to get infectious diseases, neurodegenerative diseases, cardiovascular diseases, and cancers. Moreover, our results indicate that traditional Chinese medicinal toothpaste and antibacterial toothpaste can not only inhibit the growth of pathogens but also markedly inhibit the growth of probiotics and . Therefore, the inhibitory effect of toothpaste on probiotics, together with the existence of pathogens in toothbrushes, indicates a potential risk of tooth brushing for people in a sub-healthy state.
刷牙对于维护口腔健康是必要的。但很少有研究探索牙刷中的微生物多样性，并研究这些细菌对人类健康的潜在影响。在本研究中，招募了 20 名参与者，使用高通量测序技术研究了他们口腔和牙刷中的微生物多样性。我们的结果表明，从使用中药牙膏（CB 组）和抗菌牙膏（AB 组）的参与者的牙刷中分别获得了 1136 和 976 个操作分类单元（OTUs）。在牙刷上鉴定出了病原体、和 。这些病原体的存在增加了宿主感染传染病、神经退行性疾病、心血管疾病和癌症的机会。此外，我们的结果表明，中药牙膏和抗菌牙膏不仅可以抑制病原体的生长，还可以显著抑制益生菌 和 的生长。因此，牙膏对益生菌的抑制作用以及牙刷中病原体的存在表明，对于处于亚健康状态的人来说，刷牙可能存在潜在风险。
