Horváth I, Duncan W P, Bullard J C
Acta Microbiol Acad Sci Hung. 1981;28(1):7-24.
Treponema pallidum was discovered relatively late and was not cultured in vitro. Both the delineation of T. pallidum biology and the eradication of syphilis suggest the necessity of cultivation in vitro. An attempt has been made with an improved medium to cultivate pathogenic T. pallidum Budapest strain in vitro. Only in the first passage, evidence of in vitro multiplication of T. pallidum has been established by (i) macroscopic observation, (ii) darkfield examination, (iii) electron microscopic examination, (iv) optical densities, (v) tritium labelled thymidine incorporation, and (vi) the pathogenicity off the cultured organisms was evidenced by rabbit challenge. Explanation of the oxygen utilization of T. pallidum suspension is discussed. Unidentified formations were observed on electron micrographs from the 96 h cultures. They may belong to the multiplication forms of treponemes. Further experiments are needed for their identification and for expansion of the multiplication of T. pallidum beyond the first passage.
梅毒螺旋体发现得相对较晚，且无法在体外培养。梅毒螺旋体生物学特性的描述以及梅毒的根除都表明了体外培养的必要性。人们尝试使用改良培养基在体外培养致病性梅毒螺旋体布达佩斯菌株。仅在第一代传代中，通过以下方式证实了梅毒螺旋体在体外的增殖：（i）宏观观察，（ii）暗视野检查，（iii）电子显微镜检查，（iv）光密度，（v）氚标记胸腺嘧啶核苷掺入，以及（vi）通过兔攻毒证明培养物的致病性。讨论了梅毒螺旋体悬液的氧利用情况。在96小时培养物的电子显微镜照片上观察到了不明结构。它们可能属于密螺旋体的增殖形式。需要进一步实验来鉴定它们，并使梅毒螺旋体的增殖超过第一代传代。
