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在微载体珠上培养棉尾兔表皮(Sf1Ep)细胞及其在梅毒螺旋体苍白亚种悬浮培养中的应用。

Cultivation of cottontail rabbit epidermal (Sf1Ep) cells on microcarrier beads and their use for suspension cultivation of Treponema pallidum subsp. pallidum.

作者信息

Riley B S, Cox D L

机构信息

Department of Microbiology and Immunology, Texas College of Osteopathic Medicine, Fort Worth 76107.

出版信息

Appl Environ Microbiol. 1988 Nov;54(11):2862-5. doi: 10.1128/aem.54.11.2862-2865.1988.

DOI:10.1128/aem.54.11.2862-2865.1988
PMID:3063209
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC204387/
Abstract

In vitro propagation of Treponema pallidum can be achieved by cocultivation with Sf1Ep cells. This study had two objectives: (i) to achieve suspension cultivation of Sf1Ep cells and (ii) to develop procedures for achieving the replication of T. pallidum in those cell cultures. Seven suspension cultures of Sf1Ep cells yielded an average of 7.2 x 10(8) T. pallidum (36-fold increase) after 12 days.

摘要

通过与Sf1Ep细胞共培养可实现梅毒螺旋体的体外增殖。本研究有两个目标:(i)实现Sf1Ep细胞的悬浮培养,以及(ii)开发在这些细胞培养物中实现梅毒螺旋体复制的程序。7种Sf1Ep细胞悬浮培养物在12天后平均产生了7.2×10⁸个梅毒螺旋体(增加了36倍)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dce8/204387/4c9c4d542339/aem00116-0276-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dce8/204387/4c9c4d542339/aem00116-0276-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dce8/204387/4c9c4d542339/aem00116-0276-a.jpg

相似文献

1
Cultivation of cottontail rabbit epidermal (Sf1Ep) cells on microcarrier beads and their use for suspension cultivation of Treponema pallidum subsp. pallidum.在微载体珠上培养棉尾兔表皮(Sf1Ep)细胞及其在梅毒螺旋体苍白亚种悬浮培养中的应用。
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本文引用的文献

1
In vitro cultivation of Treponema pallidum: independent confirmation.梅毒螺旋体的体外培养:独立验证
Infect Immun. 1982 Apr;36(1):437-9. doi: 10.1128/iai.36.1.437-439.1982.
2
Further studies on replication of virulent Treponema pallidum in tissue cultures of Sf1Ep cells.关于毒力梅毒螺旋体在Sf1Ep细胞组织培养中复制的进一步研究。
Infect Immun. 1982 Feb;35(2):449-55. doi: 10.1128/iai.35.2.449-455.1982.
3
Cultivation of virulent Treponema pallidum in tissue culture.在组织培养中培养毒性梅毒螺旋体。
J Bacteriol. 2015 Apr;197(7):1164-72. doi: 10.1128/JB.02472-14. Epub 2015 Jan 20.
Infect Immun. 1981 May;32(2):908-15. doi: 10.1128/iai.32.2.908-915.1981.
4
Optimizing culture conditions for the production of animal cells in microcarrier culture.优化微载体培养中动物细胞生产的培养条件。
Ann N Y Acad Sci. 1981;369:33-46. doi: 10.1111/j.1749-6632.1981.tb14175.x.
5
Propagation of poliovirus in microcarrier cultures of three monkey kidney cell lines.脊髓灰质炎病毒在三种猴肾细胞系微载体培养物中的增殖
J Biol Stand. 1981;9(2):137-45. doi: 10.1016/s0092-1157(81)80018-2.
6
Production of cell-derived products: virus and interferon.细胞衍生产品的生产:病毒与干扰素。
Ann N Y Acad Sci. 1981;369:47-59. doi: 10.1111/j.1749-6632.1981.tb14176.x.
7
Cell growth optimization in microcarrier culture.微载体培养中的细胞生长优化
In Vitro. 1980 Oct;16(10):859-65. doi: 10.1007/BF02619423.
8
Culture of respiratory syncytial virus infected diploid bovine nasal mucosa cells on cytodex 3 microcarriers.在Cytodex 3微载体上培养呼吸道合胞病毒感染的二倍体牛鼻黏膜细胞。
Arch Virol. 1986;89(1-4):81-8. doi: 10.1007/BF01309881.
9
Epidermal cell culture using Sephadex beads coated with denatured collagen (cytodex 3).使用涂有变性胶原蛋白的葡聚糖凝胶珠(细胞培养珠3)进行表皮细胞培养。
J Invest Dermatol. 1987 Jan;88(1):33-6. doi: 10.1111/1523-1747.ep12464838.
10
Prolonged survival of virulent Treponema pallidum (Nichols strain) in cell-free and tissue culture systems.毒性梅毒螺旋体(Nichols株)在无细胞和组织培养系统中的长期存活。
Infect Immun. 1977 Oct;18(1):173-82. doi: 10.1128/iai.18.1.173-182.1977.