在微载体珠上培养棉尾兔表皮（Sf1Ep）细胞及其在梅毒螺旋体苍白亚种悬浮培养中的应用。
Cultivation of cottontail rabbit epidermal (Sf1Ep) cells on microcarrier beads and their use for suspension cultivation of Treponema pallidum subsp. pallidum.
作者信息
Riley B S, Cox D L
机构信息
Department of Microbiology and Immunology, Texas College of Osteopathic Medicine, Fort Worth 76107.
出版信息
Appl Environ Microbiol. 1988 Nov;54(11):2862-5. doi: 10.1128/aem.54.11.2862-2865.1988.
Abstract
In vitro propagation of Treponema pallidum can be achieved by cocultivation with Sf1Ep cells. This study had two objectives: (i) to achieve suspension cultivation of Sf1Ep cells and (ii) to develop procedures for achieving the replication of T. pallidum in those cell cultures. Seven suspension cultures of Sf1Ep cells yielded an average of 7.2 x 10(8) T. pallidum (36-fold increase) after 12 days.
摘要
通过与Sf1Ep细胞共培养可实现梅毒螺旋体的体外增殖。本研究有两个目标：（i）实现Sf1Ep细胞的悬浮培养，以及（ii）开发在这些细胞培养物中实现梅毒螺旋体复制的程序。7种Sf1Ep细胞悬浮培养物在12天后平均产生了7.2×10⁸个梅毒螺旋体（增加了36倍）。
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