Springer W T, Truman R W
Avian Dis. 1981 Apr-Jun;25(2):528-33.
A localized complement-dependent hemolytic assay in modified Cunningham chambers for enumerating avian immunoglobulin-M (IgM)-secreting spleen cells is defined. The maximum number of IgM-secreting spleen cells was present six days after sheep red blood cells (SRBCs) were injected. Intra-abdominal injection of SRBCs resulted in greater but more variable numbers of IgM-secreting cells at the time of maximum response than did either intramuscular or intravenous injection. Fresh chicken serum (FCS) with 10% guinea pig serum was an equally or more effective source of complement for the assay than was FCS alone.
定义了一种在改良坎宁安小室中进行的局部补体依赖性溶血试验，用于计数分泌禽免疫球蛋白M（IgM）的脾细胞。在注射绵羊红细胞（SRBC）后六天出现分泌IgM的脾细胞数量最多。腹腔注射SRBC在最大反应时产生的分泌IgM的细胞数量比肌肉注射或静脉注射更多，但变化更大。含有10%豚鼠血清的新鲜鸡血清（FCS）作为该试验的补体来源，与单独使用FCS相比，同样有效或更有效。
