Sawada T, Bito Y
Immunology. 1980 Oct;41(2):331-7.
Chicken B cells treated with diluted anti-bursa cell serum in the presence of complement were transferred together with normal T cells and sheep red blood cells into immunodeficient recipient chickens, and spleen cells taken from these were examined for the development of plaque-forming cells. The production of IgM-plaque-forming cells was considerably more resistant to the cytotoxic effect of the antiserum than that of IgG-plaque-forming cells. Cells producing IgM antibodies were also less susceptible to the cytotoxic effect of the antiserum and complement than were those producing IgG antibodies. Such differential susceptibility of development of IgM- and IgG- plaque-forming cells was observed only in chickens aged about 2 weeks or younger. These findings indicate the existence of a B-cell subpopulation which is relatively insusceptible to the cytotoxic effect of the antiserum and is incapable of switching from IgM- to IgG-immune responses. The B cells resistant to the cytotoxic effect of diluted anti-bursa cell serum and complement were more susceptible to X-irradiation than were normal B cells. Immune responses against sheep red blood cells by the B cells resistant to the cytotoxic effect were dependent upon the help of T cells.
在补体存在的情况下，用稀释的抗法氏囊细胞血清处理鸡B细胞，然后将其与正常T细胞和绵羊红细胞一起转移到免疫缺陷的受体鸡中，并检查从这些鸡中取出的脾细胞中形成空斑细胞的发育情况。与IgG形成空斑细胞相比，IgM形成空斑细胞的产生对抗血清的细胞毒性作用具有更强的抗性。产生IgM抗体的细胞也比产生IgG抗体的细胞更不易受到抗血清和补体的细胞毒性作用。仅在约2周龄或更小的鸡中观察到IgM和IgG形成空斑细胞发育的这种差异敏感性。这些发现表明存在一个B细胞亚群，其对抗血清的细胞毒性作用相对不敏感，并且不能从IgM免疫反应转换为IgG免疫反应。对稀释的抗法氏囊细胞血清和补体的细胞毒性作用具有抗性的B细胞比正常B细胞对X射线更敏感。对细胞毒性作用具有抗性的B细胞对绵羊红细胞的免疫反应依赖于T细胞的帮助。
