间接免疫荧光法与酶联免疫吸附测定法检测弓形虫抗体的比较
Comparison of indirect immunofluorescence and enzyme-linked immunosorbent assay for detection of toxoplasma antibody.
作者信息
Teti G, Leonardi M S, Gazzara D
出版信息
Boll Soc Ital Biol Sper. 1981 Mar 15;57(5):561-7.
PMID:7020722
Abstract
Eighty samples of human serum were tested for antibody to Toxoplasma gondii by enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence (IIF). Development of ELISA suggested that accurate results could only be obtained with serial serum dilutions. ELISA and IIF tests showed a significant degree of correlation, but ELISA was found to be more sensitive: 17% of 24 sera showing negative IIF values showed slightly positive ELISA titres.
摘要
通过酶联免疫吸附测定（ELISA）和间接免疫荧光法（IIF）对80份人血清样本进行了弓形虫抗体检测。ELISA方法的发展表明，只有通过血清系列稀释才能获得准确结果。ELISA和IIF检测显示出显著的相关性，但发现ELISA更敏感：在24份IIF检测结果为阴性的血清中，有17%的血清ELISA检测呈弱阳性。
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