人绒毛膜促性腺激素的顺序竞争性酶免疫测定法。

Sequential competitive enzyme immunoassay for human chorionic gonadotropin.

作者信息

Tomoda S, Hamada K, Sugawa T, Takahashi K P

出版信息

Endocrinol Jpn. 1980 Dec;27(6):785-8. doi: 10.1507/endocrj1954.27.785.

DOI:10.1507/endocrj1954.27.785

Abstract

In the previous report, we had developed a simultaneous competitive enzyme immunoassay for hCG, using sheep red blood cells as the solid phase. The serum sample was not checked because of serum interference. In this report we have developed sequential competitive enzyme immunoassay. The sequential method is preferred because serum interference is removed and silicone rods are used as the solid phase instead of sheep red blood cells, which would be easily washed away in that procedure. The working range of this procedure is from 0.5 mIU/ml to 200 mIU/ml. That value for serum hCG correlates well with that by RIA (n = 0.904) Coefficients of variation are satisfied (14.2%: within assay and 15.2%: between assay).

摘要

在之前的报告中，我们开发了一种以绵羊红细胞为固相的人绒毛膜促性腺激素（hCG）同步竞争性酶免疫测定法。由于血清干扰，未对血清样本进行检测。在本报告中，我们开发了序贯竞争性酶免疫测定法。序贯法更可取，因为它消除了血清干扰，并且使用硅棒作为固相，而不是绵羊红细胞，后者在该过程中容易被冲走。该方法的工作范围为0.5 mIU/ml至200 mIU/ml。血清hCG的该值与放射免疫分析（RIA）的值相关性良好（n = 0.904）。变异系数令人满意（批内为14.2%，批间为15.2%）。