Hill J H, Bryant G R, Durand D P
J Virol Methods. 1981 Jul;3(1):27-35. doi: 10.1016/0166-0934(81)90019-7.
An optimized ELISA system for the detection of soybean mosaic virus was developed. Purified anti-soybean mosaic virus IgG, a secondary ovalbumin coat, and a clearly defined optimal concentration of enzyme-conjugated anti-soybean mosaic virus IgG are used. The test detects purified virus, or purified virus added to leaf and seed extracts, at a concentration of 2.5 ng/ml. Various buffers significantly affect detection sensitivity.
开发了一种用于检测大豆花叶病毒的优化酶联免疫吸附测定（ELISA）系统。使用了纯化的抗大豆花叶病毒IgG、二级卵清蛋白包被以及明确定义的最佳浓度的酶联抗大豆花叶病毒IgG。该检测方法能检测浓度为2.5 ng/ml的纯化病毒，或添加到叶片和种子提取物中的纯化病毒。各种缓冲液对检测灵敏度有显著影响。
