Hoidal J R, Schmeling D, Peterson P K
J Infect Dis. 1981 Jul;144(1):61-71. doi: 10.1093/infdis/144.1.61.
Phagocytic and bactericidal activities of purified polymorphonuclear neutrophils (PMNs), monocytes (MNs), or alveolar macrophages (AMs) from the same individuals for Staphylococcus aureus strain 502A or Escherichia coli strain ON2 were compared in vitro. Factors that may influence these activities were evaluated. The bactericidal activity of S. aureus and E. coli of PMNs was greater than that of MNs or AMs. Two factors were identified that may account for this result. (1) The rate of phagocytosis of opsonized S. aureus and E. coli was greater by PMNs. (2) The nonmitochondrial oxygen burst after nonparticulate stimulation was much greater by PMNs. Qualitative differences in opsonic requirements for ingestion of S. aureus by PMNs, MNs, and AMs were also identified. In contrast to PMNs or MNs, phagocytosis of S. aureus by AMs was not opsonin-dependent. The results suggest that mononuclear phagocytes may adapt their opsonic requirements to facilitate uptake of S. aureus. Despite this adaptation, their bactericidal capability remains limited when compared with PMNs.
在体外比较了来自同一受试者的纯化多形核中性粒细胞（PMN）、单核细胞（MN）或肺泡巨噬细胞（AM）对金黄色葡萄球菌502A株或大肠杆菌ON2株的吞噬和杀菌活性。评估了可能影响这些活性的因素。PMN对金黄色葡萄球菌和大肠杆菌的杀菌活性高于MN或AM。确定了两个可能解释这一结果的因素。（1）PMN对调理素化的金黄色葡萄球菌和大肠杆菌的吞噬率更高。（2）PMN在非颗粒刺激后的非线粒体氧爆发要大得多。还确定了PMN、MN和AM摄取金黄色葡萄球菌时在调理素需求方面的定性差异。与PMN或MN不同，AM对金黄色葡萄球菌的吞噬不依赖调理素。结果表明，单核吞噬细胞可能会调整其调理素需求以促进对金黄色葡萄球菌的摄取。尽管有这种适应性变化，但与PMN相比，它们的杀菌能力仍然有限。
