Ronai Z, Avraham H, Sulitzeanu D
J Parasitol. 1981 Jun;67(3):351-4.
A radio-iodinated Protein A (125SPA) binding assay was used to identify autoantibodies to red blood cells (RBC's) in sera of rats infected with Plasmodium berghei. Sera taken from rats at various times after infection were reacted with washed, normal RBC's, then the RBC's were washed and treated with 125SPA. The bound radioactivity was taken as a measure of the amount of Ig attached to the RBC's membranes. Using this test, anti-RBC autoantibodies were detected in rat sera as early as 5 days after infection. The level of the autoantibodies rose to a maximum at 12 to 14 days, at which time parasitemia was also maximal, then declined sharply. Autoantibodies were still detectable at 21 days after infection. Red blood cells from infected rats had readily detectable, membrane-bound Ig, as shown by their capacity to bind 125SPA directly. The amount of this Ig rose and fell in a fashion closely paralleling course of parasitemia.
采用放射性碘化蛋白A（125SPA）结合试验来鉴定感染伯氏疟原虫的大鼠血清中针对红细胞（RBC）的自身抗体。在感染后的不同时间采集大鼠血清，使其与洗涤过的正常RBC反应，然后洗涤RBC并使用125SPA进行处理。结合的放射性被视为附着在RBC膜上的Ig量的指标。通过该试验，在感染后5天就检测到大鼠血清中的抗RBC自身抗体。自身抗体水平在12至14天升至最高，此时寄生虫血症也最高，然后急剧下降。感染后21天仍可检测到自身抗体。感染大鼠的红细胞具有易于检测到的膜结合Ig，这可通过它们直接结合125SPA的能力来证明。这种Ig的量随着寄生虫血症的进程呈平行的上升和下降趋势。
