Berzins K, Wahlgren M, Perlmann P
Clin Exp Immunol. 1983 Nov;54(2):313-8.
Sera from patients with Plasmodium falciparum, P. vivax or P. ovale malaria were selected according to their high levels of antibodies against human erythrocyte membranes as measured in a microELISA. The specificity of the anti-erythrocyte antibodies in these sera and two normal sera was investigated by means of an immunoblotting technique in combination with SDS-polyacrylamide gel electrophoresis. All the patients' sera as well as the control sera contained antibodies against several erythrocyte polypeptides. As compared with normal sera, most malaria sera showed elevated levels of antibodies against polypeptides of 80K, 70K, 40K and 28K molecular weights. Two sera reacted strongly against a polypeptide with an electrophoretic mobility similar to the alpha subunit of spectrin. One serum showed strong reaction and several other sera, including normal sera, showed weak reaction against a 45K molecular weight polypeptide corresponding to actin. No pervading differences were seen in the pattern of specificities of the anti-erythrocyte ghost antibodies between sera from patients with P. falciparum, P. vivax or P. ovale infections.
根据微量酶联免疫吸附测定法检测结果，选择恶性疟原虫、间日疟原虫或卵形疟原虫疟疾患者的血清，这些患者血清中针对人红细胞膜的抗体水平较高。采用免疫印迹技术结合十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳，研究这些血清以及两份正常血清中抗红细胞抗体的特异性。所有患者血清以及对照血清均含有针对几种红细胞多肽的抗体。与正常血清相比，大多数疟疾血清中针对分子量为80K、70K、40K和28K的多肽的抗体水平升高。两份血清与一种电泳迁移率类似于血影蛋白α亚基的多肽发生强烈反应。一份血清与对应于肌动蛋白的45K分子量多肽发生强烈反应，其他几份血清（包括正常血清）则发生较弱反应。在恶性疟原虫、间日疟原虫或卵形疟原虫感染患者的血清中，抗红细胞血影抗体的特异性模式未见普遍差异。
