Pontes J E, Rose N R, Ercole C, Pierce J M
J Urol. 1981 Aug;126(2):187-9. doi: 10.1016/s0022-5347(17)54440-7.
An indirect immunofluorescence technique to detect cells producing prostatic acid phosphatase was used to evaluate 12 biopsies of soft tissue masses of possible metastatic prostatic carcinoma. In 10 patients varying degrees of immunofluorescence were observed, confirming the origin of the primary tumor. Specimens from 34 patients with prostatic carcinoma were obtained either by radical prostatectomy or transurethral resection of the prostate. A comparison of histological grade (Gleason), semiquantitative immunofluorescence for prostatic acid phosphatase and hormonal responsiveness was done. There was a suggestion of higher hormonal responsiveness in the group with a low Gleason score and a high percentage of positive immunofluorescence that presently cannot be evaluated completely since some patients still are under therapy. This method may be used in the future in conjunction with other techniques, such as androgen receptors, to define a population of patients most likely to respond to hormonal manipulation.
一种用于检测产生前列腺酸性磷酸酶细胞的间接免疫荧光技术，被用于评估12例可能为转移性前列腺癌的软组织肿块活检样本。在10例患者中观察到了不同程度的免疫荧光，证实了原发肿瘤的来源。34例前列腺癌患者的样本通过根治性前列腺切除术或经尿道前列腺切除术获取。对组织学分级（Gleason）、前列腺酸性磷酸酶的半定量免疫荧光以及激素反应性进行了比较。在Gleason评分低且免疫荧光阳性百分比高的组中，提示有更高的激素反应性，由于一些患者仍在接受治疗，目前尚无法完全评估。这种方法未来可能会与其他技术（如雄激素受体）结合使用，以确定最有可能对激素治疗产生反应的患者群体。
