Collins A B, Andres G A, McCluskey R T
Nephron. 1981;27(6):297-301. doi: 10.1159/000182074.
Renal tissue from 71 patients with membranous glomerulonephritis was studied for the presence of renal tubular antigens in glomerular deposits by indirect immunofluorescence, using a rabbit antiserum prepared against a human renal tubular antigen, Fx1A. The rabbit antiserum was reactive with antigens normally present in the brush border regions of proximal tubules of human and rat kidneys and in the glomerular deposits of rats with Heymann nephritis. However, renal tubular antigens were not detectable in any of the cases of human membranous glomerulonephritis, either before or after partial elution of immunoglobulins from glomeruli. Eluates of renal tissue from 5 of the patients and sera from 6 patients did not contain antibodies against renal tubular antigens demonstrable by immunofluorescence. These findings fail to provide evidence for a role of renal tubular antigens in human membranous glomerulonephritis.
采用针对人肾小管抗原Fx1A制备的兔抗血清，通过间接免疫荧光法研究了71例膜性肾小球肾炎患者的肾组织，以检测肾小球沉积物中肾小管抗原的存在情况。该兔抗血清可与人及大鼠肾脏近端小管刷状缘区域正常存在的抗原以及患海曼肾炎大鼠的肾小球沉积物中的抗原发生反应。然而，在人膜性肾小球肾炎的任何病例中，无论是在从肾小球部分洗脱免疫球蛋白之前还是之后，均未检测到肾小管抗原。5例患者的肾组织洗脱液和6例患者的血清中均未含有通过免疫荧光法可证实的抗肾小管抗原抗体。这些发现未能为肾小管抗原在人膜性肾小球肾炎中的作用提供证据。
